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AssigneeOxford Gene Technology IP Limited
InventorsEdwin Mellor Southern, Mikhail Sergeevich Shchepinov, John Nicholas Housby, Alan Lewis Hamilton, John Kenneth Elder
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A method of making a set of labelled compounds by the use of a preferably particulate support, comprises dividing the support into lots, performing a different chemical reaction on each lot of the support, e.g. to couple a chemical moiety to that lot of the support, tagging a fraction of each lot of the support with a different label, and combining the said lots of the support. The steps are repeated several times, preferably to build up oligomer molecules carrying labels which identify the nature and position of a monomer unit of the oligomer, and which are releasable from the support. Preferred labels, which are releasable from the compounds by cleavage to provide charged groups for analysis by mass spectrometry, are groups of the trityl (trimethylphenyl) family. Also claimed are libraries of these labels and their use in assays and nucleic acid analysis methods.
A method, which method comprises providing a labelled oligonucleotide or nucleic acid, and removing the label by cleavage to give a charged species which is subjected to mass spectrometry, wherein the label is a group of formula R1R2R3C—, where R1, R2 and R3 are the same or different and each is a monocyclic or fused ring aromatic group that is substituted or unsubstituted.
An assay method in which a labelled probe is partitioned into two fractions one of which is determined, the probe comprising a ligand joined to a label by a link which is cleavable to give a charged species for mass spectrometry, wherein the label is a group of formula R1R2R3C—, where R1, R2 and R3 are the same or different and each is a monocyclic or fused ring aromatic group that is substituted or unsubstituted.
Dependencies are inferred from the claim text.