Method for preparing a DNA chip and use thereof

A method for producing a DNA chip, the method comprising the steps of:

• (a) ligating one or more double-helical probes into one or more vectors, wherein each probe comprises an oligonucleotide coupled to a linker at one or both ends, and wherein the oligonucleotide is a 60 bp by sequence specific to an HPV genotype, where a linker is coupled to both ends of an oligonucleotide to be integrated on a slide, into a vector;
• (b) transforming host cells with the vectors;
• (c) culturing the transformed host cells to recover a plurality of double-helical probes, each including the linker, wherein the linker is a part of the base sequence of the vector;
• and
• (d) integrating the plurality of double-helical probes on a slide to produce a DNA chip, wherein the double-helical probes of said plurality of double helical probes are selected from the group consisting of:
• (i) SEQ ID NOs:
• 1 and 2;
• (ii) SEQ ID NOs:
• 3 and 4;
• (iii) SEQ ID NOs:
• 5 and 6;
• (iv) SEQ ID NOs:
• 7 and 8;
• (v) SEQ ID NOs:
• 9 and 10;
• (vi) SEQ ID NOs:
• 11 and 12;
• (vii) SEQ ID NOs:
• 13 and 14;
• (viii) SEQ ID NOs:
• 15 and 16;
• (ix) SEQ ID NOs:
• 17 and 18;
• (x) SEQ ID NOs:
• 19 and 20;
• (xii) SEQ ID NOs:
• 21 and 22;
• (xiii) SEQ ID NOs:
• 23 and 24;
• (xiv) SEQ ID NOs:
• 25 and 26;
• (xv) SEQ ID NOs:
• 27 and 28;
• (xvi) SEQ ID NOs:
• 29 and 30;
• (xvii) SEQ ID NOs:
• 31 and 32;
• (xviii) SEQ ID NOs:
• 33 and 34;
• (xix) SEQ ID NOs:
• 35 and 36;
• (xx) SEQ ID NOs:
• 37 and 38;
• (xxi) SEQ ID NOs:
• 39 and 40;
• (xxii) SEQ ID NOs:
• 41 and 42;
• (xxiii) SEQ ID NOs:
• 43 and 44;
• (xxiv) SEQ ID NOs:
• 45 and 46;
• (xxv) SEQ ID NOs:
• 47 and 48;
• (xxvi) SEQ ID NOs:
• 49 and 50;
• (xxvii) SEQ ID NOs:
• 51 and 52;
• and
• (xxviii) SEQ ID NOs:
• 53 and 54;
• and wherein the DNA chip is effective in the identification of Human Papilloma Virus (HPV) infection and the genotype of HPV infection in a sample.