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US 7,741,039 B2Granted
Postweaning multisystemic wasting syndrome virus from pigs
AssigneeMerial Limited
InventorsLi Wang, Lorne A. Babiuk, Andrew A. Potter, Philip Willson
Life of this patent
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Abstract
The cloning of a novel PCVII viral genome is described as is expression of proteins derived from the PCVII genome. These proteins can be used in vaccine compositions for the prevention and treatment of PCVII infections, as well as in diagnostic methods for determining the presence of PCVII infections in a vertebrate subject. Polynucleotides derived from the viral genome can be used as diagnostic primers and probes.
Claims7
A method for detecting PCVII homologous sequences in a biological sample comprising:
- (a) incubating the biological sample with a polynucleotide that specifically hybridizes to a PCVII nucleotide sequence having SEQ ID NO:
- 11 or SEQ ID NO:
- 12 but not to polynucleotides from other members of the circovirus family, wherein the polynucleotide comprises at least 12 contiguous nucleotides from, or complementary to a PCVII genomic sequence, under conditions of high stringency;
- and
- (b) detecting the complexes containing the polynucleotide.
2 more elements
A method for detecting PCVII homologous sequences in a biological sample comprising:
- (a) incubating the biological sample with a polynucleotide that specifically hybridizes to a PCVII nucleotide sequence having SEQ ID NO:
- 1, but not to polynucleotides from other members of the circovirus family, wherein the polynucleotide comprises at least 12 contiguous nucleotides from, or complementary to a PCVII genomic sequence, under conditions which promote the formation of nucleic acid complexes between the polynucleotide and the PCVII nucleotide sequence present in the biological sample;
- and
- (b) detecting the complexes containing the polynucleotide.
1 more element
Dependencies are inferred from the claim text.