Protein inhibitor of Ran activity and methods of use thereof
AssigneeWisconsin Alumi Research Foundation
InventorsFrederick William Porter, Ann Carol Palmenberg, Christiane Wiese, Yury Alexandrovitch Bochkov
Life of this patent
Estimating the term…
Abstract
The invention provides a method of inhibiting Ran protein activity in at least one eukaryotic cell or cell-free extract, the method comprising exposing an amino acid sequence comprising at least a portion of EMCV or TMEV leader protein, wherein the amino acid sequence comprises SEQ. ID NO: 14 or SEQ. ID NO: 15, to at least one cell in an amount effective to inhibit Ran activity in the targeted cell and evaluating Ran protein activity in the cell.
Claims11
A method of inhibiting Ran protein activity in at least one eukaryotic cell or cell-free extract, the method comprising:
- (a) exposing an amino acid sequence comprising a purified full-length encephalomyocarditis virus (EMCV) leader protein (L protein) to at least one cell or cell-free extract in an amount effective to inhibit Ran activity in the cell or cell-free extract;
- and
- (b) evaluating Ran protein activity in the cell or cell-free extract.
A method of inhibiting Ran protein activity in a eukaryotic cell, the method comprising administering to a patient in need thereof a therapeutically effective amount of an amino acid sequence comprising full-length encephalomyocarditis virus (EMCV) leader protein, and determining the effect of inhibiting Ran activity in the patient.
A method of inhibiting Ran protein activity in at least one eukaryotic cell or cell-free extract, the method comprising:
- (a) exposing an amino acid sequence comprising a modified full-length encephalomyocarditis virus (EMCV) leader protein (L protein) which has been purified to at least one cell or cell-free extract in an amount effective to inhibit Ran activity in the cell or cell-free extract, wherein said modification consists of deleting the acidic domain from the full-length protein;
- and
- (b) evaluating Ran protein activity in the cell or cell-free extract.
Dependencies are inferred from the claim text.