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US 7,741,018 B2Granted
Advantageous vitrifiable cryoprotectant mixtures
Assignee21st Century Medicine, Inc.
InventorGregory M. Fahy
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Abstract
Disclosed herein are aqueous carrier solutions for cryoprotectants that are useful for a variety of cryoprotectant solutions for use with cells, tissues, and whole organs, said aqueous carrier solutions permitting antinucleators to be fully effective in vitrification solutions, thereby allowing vitrification solutions to attain extreme effectiveness, and compatible vitrification solution compositions for use with these carrier solutions. The carrier solutions comprise lactose, mannitol or trehalose, and glucose in combination with other beneficial ingredients.
Claims5
A cryoprotectant mixture comprising at least:
- dimethyl sulfoxide (DMSO),
- formamide, and
- ethylene glycol (EG);
- wherein said cryoprotectant mixture is selected from the group consisting of:
- 22% DMSO+13% formamide+24% EG+1% X1000+4% decaglycerol;
- 22% DMSO+13% formamide+17% EG+7% aectol+1% X1000+4% decaglycerol;
- 22% DMSO+13% formamide+17% EG+1% X1000+1% decaglycerol+7% polyvinylpyrrolidone;
- 22% DMSO+13% formamide+17% EG 0.1% X1000+2.9% decaglycerol+7% polyvinylpyrrolidone;
- 21% DMSO+17% formamide+17% EG+0.5% X1000+0.5% decaglycerol+6% polyvinylpyrrolidone;
- 22% DMSO+12% formamide+17% EG+0.5% X1000+0.5% decaglycerol+7% polyvinylpyrrolidone;
- 23% DMSO+13% formamide+16% EG+0.5% X1000+0.5% decaglycerol+6% polyvinylpyrrolidone;
- 22% DMSO+13.2% formamide 16.8% EG+1% X1000+1% decaglycerol+7% polyvinylpyrrolidone;
- 22% DMSO+13% formamide+17% EG+0.1% X1000+1.9% decaglycerol+7% polyvinylpyrrolidone;
- and
- 22% DMSO+13% formamide+17% EG+1% X1000+2% decaglycerol+7% polyvinylpyrrolidone.
12 more elements
Dependencies are inferred from the claim text.