Back to results
US 7,740,867 B2Granted
Method for identification of virulence determinants
AssigneeBoard of Regents of the University of Nebraska
InventorsRaul G. Barletta, N. Beth Harris
Life of this patent
Estimating the term…
Abstract
Disclosed are methods for the determination of virulence determinants in bacteria and in particular bacteria of the genus Mycobacterium. Also disclosed are compositions and methods for stimulating an immune response in an animal using bacteria and virulence determinants identified by the methods of the present invention.
Claims10
A method for isolating a bacteria comprising:
- introducing at least one mutation into the genome of a virulent bacteria;
- culturing the mutated bacteria in the presence of an antimicrobial agent that kills growing but not non-growing bacteria;
- selecting surviving bacteria;
- testing the selected surviving bacteria for virulence;
- and
- selecting attenuated mutant bacteria.
3 more elements
Dependencies are inferred from the claim text.