Drug delivery product and methods
AssigneeUniversity of Massachusetts
InventorGary R. Ostroff
Life of this patent
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Abstract
The present invention provides a particulate delivery system comprising an extracted yeast cell wall comprising beta-glucan, a payload molecule and a payload trapping molecule. The invention further provides methods of making and methods of using the particulate delivery system.
Claims55
A particulate delivery system comprising an extracted yeast cell wall comprising less than 90 weight percent beta-glucan and a payload trapping molecule, wherein the payload trapping molecule is contained within an interior space defined by the extracted yeast cell wall and wherein the payload trapping molecule is present in an amount sufficient to facilitate retention of a payload within the extracted yeast cell wall.
An article of manufacture comprising a first container containing a payload molecule selected from the group consisting of a nucleic acid composition, protein composition, small organic molecule and mixtures thereof, a second container containing a particulate delivery system wherein the particulate delivery system comprises an extracted yeast cell wall comprising less than 90 weight percent beta-glucan and a payload trapping molecule and instructions for use, wherein the payload trapping molecule is contained within an interior space defined by the extracted yeast cell wall and wherein the payload trapping molecule is present in an amount sufficient to facilitate retention of the payload molecule within the extracted yeast cell wall.
A pharmaceutical composition comprising a particulate delivery system wherein the particulate delivery system comprises an extracted yeast cell wall comprising less than 90 weight percent beta-glucan, a payload trapping molecule and a payload molecule selected from the group consisting of a polynucleotide, a protein, a small organic molecule and mixtures thereof, and a pharmaceutically acceptable excipient, wherein the payload trapping molecule is contained within an interior space defined by the extracted yeast cell wall and wherein the payload trapping molecule is present in an amount sufficient to facilitate retention of the payload molecule within the extracted yeast cell wall.
A method of delivering a payload molecule to a cell comprising the steps of:
- providing an extracted yeast cell wall comprising less than 90 weight percent beta-glucan, the yeast cell wall defining an internal space;
- contacting the extracted yeast cell wall with a payload molecule wherein the payload molecule becomes at least partially enclosed within the internal space;
- contacting the extracted yeast cell wall with a payload trapping molecule wherein the payload trapping molecule at least partially confines the payload molecule within the extracted yeast cell wall to form a particulate delivery system, wherein the payload trapping molecule is contained within an interior space defined by the yeast cell wall particle, wherein the payload trapping molecule is present in an amount sufficient to facilitate retention of the payload molecule within the yeast cell wall, and wherein the payload molecule and the payload trapping molecule are soluble in the same solvent system;
- and
- contacting a cell with the particulate delivery system.
A method of delivering a drug to a phagocytic cell comprising the steps of:
- providing an extracted yeast cell wall comprising less than 90 weight percent beta-glucan, the yeast cell wall defining an internal space;
- contacting the extracted yeast cell wall with a payload molecule wherein the payload molecule becomes associated with extracted yeast cell wall;
- contacting the extracted yeast cell wall with a payload trapping molecule wherein the payload trapping molecule stabilizes the association of the payload molecule and the extracted yeast cell wall to form a particulate delivery system, wherein the payload trapping molecule is contained within an interior space defined by the yeast cell wall particle, wherein the payload trapping molecule is present in an amount sufficient to facilitate retention of the payload molecule within the yeast cell wall;
- and wherein the payload molecule and the payload trapping molecule are soluble in the same solvent system;
- and
- contacting a phagocytic cell with the particulate drug delivery system.
A method of making a particulate delivery system comprising the steps of providing an extracted yeast cell wall comprising less than 90 weight percent beta-glucan, the yeast cell wall defining an internal space;
- contacting the extracted yeast cell wall with a payload molecule wherein the payload molecule becomes associated with extracted yeast cell wall;
- and contacting the extracted yeast cell wall with a payload trapping molecule wherein the payload trapping molecule stabilizes the association of the payload molecule and the extracted yeast cell wall to form a particulate delivery system, wherein the payload trapping molecule is contained within an interior space defined by the yeast cell wall particle, and wherein the payload trapping molecule is present in an amount sufficient to facilitate retention of the payload molecule within the yeast cell wall.
A method of exposing an individual to an antigen comprising the step of contacting a phagocytic cell of the individual with a particulate delivery system comprising an extracted yeast cell wall comprising less than 90 weight percent beta-glucan, a payload trapping molecule and payload molecule, wherein the payload molecule is a polynucleotide comprising a control element operatively linked to an open reading frame encoding a peptide that can be controllably expressed in the cells of the individual, wherein the payload trapping molecule is contained within an interior space defined by the yeast cell wall particle, and wherein the payload trapping molecule is present in an amount sufficient to facilitate retention of the payload molecule within the yeast cell wall.
The method of exposing an individual to an antigen comprising the step of contacting a phagocytic cell of the individual with a particulate delivery system comprising an extracted yeast cell wall comprising less than 90 weight percent beta-glucan, a payload trapping molecule and payload molecule, wherein the payload molecule is an antigenic molecule, wherein the payload trapping molecule is contained within an interior space defined by the yeast cell wall particle, and wherein the payload trapping molecule is present in an amount sufficient to facilitate retention of the payload molecule within the yeast cell wall.
A method of delivering a drug to a macrophage cell comprising the steps of:
- providing a particulate delivery system comprising less than 90 weight percent an extracted yeast cell wall comprising beta-glucan, a drug and a payload trapping molecule, wherein the payload trapping molecule is contained within an interior space defined by the yeast cell wall particle, and wherein the payload trapping molecule is present in an amount sufficient to facilitate retention of the payload molecule within the yeast cell wall;
- and
- contacting a macrophage cell with the particulate drug delivery system.
Dependencies are inferred from the claim text.