Ultra high affinity neutralizing antibodies
AssigneeMedImmune, LLC
InventorsJames F. Young, Leslie S. Johnson, William D. Huse, Herren Wu, Jeffry D. Watkins
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Abstract
Ultra high affinity antibodies with binding affinities in the range of 1010 M−1, and even 1011 M−1 are disclosed. Such antibodies include antibodies having novel high affinity complementarity determining regions (CDRs), especially those with framework and constant regions derived from either humans or mice. Methods of preparing and screening such antibodies, as well as methods of using them to prevent and/or treat disease, especially virus-induced diseases, are also disclosed.
Claims32
A method for preventing a respiratory syncytial virus (RSV)-induced disease, comprising administering to a patient a high affinity neutralizing immunoglobulin that specifically binds to a RSV F antigen with an affinity constant (Ka) of at least 1010 M−1 as measured by surface plasmon resonance, wherein the high affinity neutralizing immunoglobulin binds to the same epitope on the RSV F antigen as an antibody comprising a heavy chain variable region (VH) having an amino acid sequence SEQ ID NO:2 (FIG. 1B) and a light chain variable region (VL) having the amino acid sequence SEQ ID NO:1 (FIG. 1A).
A method for treating a respiratory syncytial virus-induced disease, comprising administering to a patient a high affinity neutralizing immunoglobulin that specifically binds to a RSV F antigen with a Ka of at least 1010 M−1 as measured by surface plasmon resonance, wherein the high affinity neutralizing immunoglobulin binds to the same epitope on the RSV F antigen as an antibody comprising a VH having the amino acid sequence SEQ ID NO:2 (FIG. 1B) and a VL having an amino acid sequence SEQ ID NO:1 (FIG. 1A).
Dependencies are inferred from the claim text.