# US 7,740,747 B2 · Injection method for microfluidic chips

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- Number: US 7,740,747 B2
- Document ID: US7740747B2
- Status: Granted
- Kind: B2
- Application number: 11/965,794
- Filed: 2007-12-28
- Published: 2009-07-02
- Granted: 2010-06-22
- Earliest claimed: 2007-12-28
- Assignees: General Electric Company
- Inventors: Wei-Cheng Tian; Erin Jean Finehout; Li Zhu; Jun Xie; Shashi Thutupalli
- Web page: https://patentgrep.com/patent/US7740747B2

## CPC

- G01N 27/44743: {Introducing samples}
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)

## Abstract

A microchip for capillary electrophoresis is provided. The microchip comprises an injection channel and a separation channel configured to receive a sample through a sample well disposed on a first end of the separation channel; wherein the injection channel and the separation channel intersect to form a ‘T’ junction. The microchip further comprises a first valve disposed adjacent to the ‘T’ junction and on the separation channel and a second valve disposed at the ‘T’ junction. The second valve is a two-way valve. A sample plug is injected into an area between the ‘T’ junction and a second end of the separation channel.

## Claims

### Claim 1

A microchip for capillary electrophoresis, the microchip comprising:
a separation channel comprising a first end and a second end, said second end being adapted to deliver an electrical potential;
an injection channel comprising a first end and configured to receive a sample through a sample well disposed on the first end of the separation channel, said first end being adapted to deliver an electrical potential; wherein the injection channel and the separation channel intersect to form a ‘T’ junction; wherein a sieving matrix occupies a region after the ‘T’ junction;
a first valve disposed adjacent to the ‘T’ junction and on the separation channel between the ‘T’ junction and the first end of the separation channel and adapted to deliver an electrical potential; and
a second valve disposed at the ‘T’ junction; wherein the second valve is a two-way valve; said valve being adapted to be positioned to either preclude flow between said first end of said separation channel and said second end of said separation channel or to preclude flow between said injection channel and said separation channel and to deliver an electric potential.

### Claim 2 (depends on 1)

The microchip of claim 1, wherein the first valve is open and the second valve is closed to flow between said first end of said separation channel and said second end of said separation channel.

### Claim 3 (depends on 2)

The microchip of claim 2, wherein a sieving matrix is loaded to the separation channel.

### Claim 4 (depends on 1)

The microchip of claim 1, wherein a voltage is maintained across the first valve and the second end of the separation channel and the second valve is closed to flow between said injection channel and said separation channel and the first valve is closed.

### Claim 5 (depends on 1)

The microchip of claim 1, wherein loading the sieving matrix comprises loading a predetermined amount of the sieving matrix.

### Claim 6 (depends on 1)

The microchip of claim 1, wherein the separation channel is partially loaded with a predetermined amount of the sieving matrix.

### Claim 7 (depends on 1)

The microchip claim 1, wherein a portion of the sample that injects into the separation channel is defined by an area between the first valve and the second valve.

### Claim 8

A method for electrophoresis, the method comprising:
forming an injection channel comprising a first end and configured to receive a sample through a sample well disposed on the first end of the separation channel;
forming a separation channel comprising a first end and a second end; wherein the injection channel and the separation channel intersect to form a ‘T’ junction;
disposing a first valve between a first end of the separation channel and the ‘T’ junction on the separation channel;
disposing a second valve at the ‘T’ junction; wherein the second valve is a two-way valve; said valve being adapted to be positioned to either preclude flow between said first end of said separation channel and said second end of said separation channel or to preclude flow between said injection channel and said separation channel;
loading a sample into said first end of said injection channel;
opening said first valve and operating said second valve to preclude flow between said first end of said separation channel and said second end of said separation channel and using electrical potential to draw said sample into said first end of said separation channel;
closing said first valve and operating said second valve to allow flow between said first end of said separation channel and said second end of said separation channel and to preclude flow between said injection channel and said separation channel; and
injecting a portion of the sample into an area between the ‘T’ junction and a second end of the separation channel using an electrical potential between said first valve and said second end of said separation channel.

### Claim 9 (depends on 8)

The method of claim 8, further comprising loading a sample into the separation channel by opening the first valve and closing the second valve in a vertical direction.

### Claim 10 (depends on 8)

The method of claim 8, further comprising applying a voltage across said first valve and said second end of said separation channel while simultaneously opening said second valve to allow flow between said first end of said separation channel and said second end of said separation channel and closing said first valve.

### Claim 11 (depends on 8)

The method of claim 8, wherein the step of separating comprises applying a voltage across the first valve and the second end of the separation channel while simultaneously opening the second valve to allow flow between said first end of said separation channel and said second end of said separation channel and closing the first valve.

### Claim 12 (depends on 11)

The method of claim 11, further comprising applying a pull back voltage between the first end of the separation channel and the injection channel.