Thiaminases and thiaminase genes for use in apoptotic therapies
AssigneeBrandeis University
InventorsChandler Fulton, Elaine Y. Lai
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Abstract
This disclosure describes methods for preparing and utilizing thiaminase and thiaminase genes to induce apoptosis in a selected group of vertebrate cells in vivo by reducing the level of thiamin in the cells. Emphasis is on methods for inducing apoptosis in cancer cells.
Claims6
A eukaryotic expression vector comprising a recombinant nucleic acid sequence encoding thiaminase I from N. gruberi, said recombinant nucleic acid sequence as set forth in SEQ ID NO:3.
A vector comprising a recombinant nucleic acid sequence encoding thiaminase I from N. gruberi, said recombinant nucleic acid sequence as set forth in SEQ ID NO:3.
An isolated bacterium transfected with a vector, said bacterium selected from the group consisting of avirulent C. sporogenes, avirulent C. beijerinckii, and attenuated, non-pathogenic S. typhimurium, said vector comprising a recombinant nucleic acid sequence encoding thiaminase I from N. gruberi as set forth in SEQ ID NO:3, and said recombinant nucleic acid sequence operably linked to a promoter.
Dependencies are inferred from the claim text.