Method for evaluating DNA probes position on substrate
AssigneeHitachi High-Technologies Corporation
InventorsKazuhito Rokutan, Hiroyuki Tomita, Toshiro Saito
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Abstract
An oligonucleotide array comprising an array of multiple oligonucleotides with different base sequences fixed onto known and separate positions on a support substrate, wherein said oligonucleotides are biological stress related genes or complementary sequence chains to the said genes, and the said oligonucleotides are classified according to their gene functions, wherein the fixation region on the support substrate is divided into the said classification.
Claims8
An oligonucleotide array comprising multiple subblock regions and oligonucleotides of different genes positioned to each of said multiple subblock regions, each of said multiple subblock regions having oligonucleotides positioned thereon based on at least one of gene function and gene expression,
- wherein said oligonucleotides within a subblock region are positioned according to an arrangement pattern, said pattern being an arrangement of oligonucleotides of the different genes,
- wherein the arrangement is formed from at least oligonucleotides of genes associated with each other at a first predetermined correlation degree being positioned closer to each other than oligonucleotides of genes associated with each other at a second predetermined correlation degree which is lower than the first correlation degree, and
- wherein a correlation degree between oligonucleotides of genes associated with each other is based on a classification algorithm using at least one of significant probability value (P value), fluorescent differential display (FDD) and a classification algorithm of a supervised method.
Dependencies are inferred from the claim text.