Laser microscope

A laser microscope comprising:

• an observation light path for guiding an observation laser beam;
• an optical stimulation light path for guiding an optical stimulation laser beam;
• and
• a light-path combining unit configured to combine said light paths;
• wherein the laser microscope further comprises, in at least the optical stimulation light path:
• at least one of a focal-position adjusting unit configured to adjust a focal position of the laser beam and a spot-diameter adjusting unit configured to adjust a spot diameter of the laser beam;
• an optical-axis misalignment detector configured to detect an amount of misalignment of an optical axis between at least one of the focal-position adjusting unit and the spot-diameter adjusting unit, and the light-path combining unit;
• and
• an alignment unit configured to adjust the optical axis so that irradiation positions of the observation laser beam and the optical stimulation laser beam are not relatively shifted on a plane perpendicular to the optical axis on a specimen, based on the amount of misalignment of the optical axis detected by the optical-axis misalignment detector.