Applications of adaptive optics in microscopy
AssigneeCarl Zeiss MicroImaging GmbH
InventorsRalf Wolleschensky, Robert Grub, Ulrich Simon, Martin Gluch, Andreas Faulstich, Martin Voelcker
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Abstract
The use of one or more wavefront modulators in the observation beam path and/or illumination beam path of a microscope provide various advantageous results. Such modulators may be adapted to change the phase and/or the amplitude of light in such a way to carry out displacement and shaping of the focus in the object space and correction of possible aberrations. The possible areas of use include confocal microscopy, laser-assisted microscopy, conventional light microscopy and analytic microscopy.
Claims12
A laser scanning microscope arrangement, comprising:
- a laser scanning microscope having a short pulse laser which illuminates a focused point that is scanned across an object;
- and
- at least one controllable wavefront modulator with adaptive optics for deliberately influencing phase or amplitude of a light wave to change the wavefront of the light wave and located following the laser;
- wherein the modulator is located in the pupil plane of the beam path.
A laser scanning microscope arrangement, comprising:
- a laser scanning microscope having a short pulse laser which illuminates a focused point that is scanned across an object;
- and
- at least one controllable wavefront modulator with adaptive optics for influencing phase or amplitude of a light wave to change the wavefront of the light wave, the controllable wavefront modulator being arranged in an excitation beam path and arranged to receive light from the short pulse laser;
- wherein the modulator is located in the pupil plane of the beam path.
Dependencies are inferred from the claim text.