Cotton fiber transcriptional factors
AssigneeCalgene, Inc.
InventorsKevin McBride, David M. Stalker, Julie R. Pear, Luis Perez-Grau
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Abstract
Novel DNA constructs are provided which may be used as molecular probes or inserted into a plant host to provide for modification of transcription of a DNA sequence of interest in cotton fiber, particularly in very early fiber development. The DNA constructs comprise a cotton fiber transcriptional initiation regulatory region associated with a gene which is expressed in cotton fiber.
Claims47
A recombinant DNA sequence comprising as operably joined components in the direction of transcription, a cotton fiber transcriptional initiation region functional in a cotton fiber cell and an open reading frame encoding an enzyme in a pigment biosynthesis pathway, wherein said transcriptional initiation region is selected from the group consisting of:
- a. nucleotides 65-4163 of SEQ ID NO:7, and
- b. SEQ ID NO:15.
A recombinant DNA sequence comprising as operably joined components in the direction of transcription;
- a transcriptional initiation region functional in a cotton plant cell and an open reading frame encoding an enzyme in a biosynthetic pathway of melanin or indigo, wherein said transcriptional initiation region is selected from the group consisting of:
- a. nucleotides 65-4163 of SEQ ID NO:7, and
- b. SEQ ID NO:15.
An isolated DNA sequence comprising a transcriptional initiation region functional in a cotton plant cell, wherein said transcriptional initiation region is selected from the group consisting of:
- a. nucleotides 65-4163 of SEQ ID NO:7, and
- b. SEQ ID NO:15.
An isolated DNA sequence comprising the sequence shown in SEQ ID NO:
- 1.
An isolated DNA sequence comprising the sequence shown in SEQ ID NO:
- 12.
An isolated DNA sequence comprising nucleotides 65-4163 of SEQ ID NO:
- 7.
An isolated DNA sequence comprising SEQ ID NO:
- 15.
A recombinant DNA construct comprising nucleotides 65-4163 of SEQ ID NO:
- 7.
A recombinant DNA construct comprising SEQ ID NO:
- 15.
A method of modifying fiber color in a cotton plant, said method comprising:
- transforming a cotton plant cell with a DNA construct comprising a DNA sequence comprising I) a sequence selected from the group consisting of SEQ ID NO:15 and nucleotides 65-4163 of SEQ ID NO:7, and ii) an open reading frame encoding a protein selected from the group consisting of tyrosinase, tryptophanase and indole oxygenase;
- regenerating a cotton plant comprising fiber tissue from said cotton plant cell, wherein said fiber tissue comprises a substrate of said protein, and wherein said protein reacts with said substrate to produce a pigment, whereby the color of said fiber is modified.
Dependencies are inferred from the claim text.