O-methyltransferase gene from sorghum cloning, expression, transformation and characterization
AssigneeThe United States of America as represented by the Secretary of Agriculture
InventorsScott R. Baerson, Agnes M. Rimando, Franck E. Dayan, Zhiqiang Pan, James J. Polashock
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Abstract
This invention relates to an O-methyltransferase gene cloned from sorghum, the sorghum O-methyltransferase-3 gene, SbOMT3. Quantitative real-time RT-PCR and recombinant enzyme studies with putative O-methyltransferase sequences obtained from an EST data set from sorghum have led to the identification of the novel root hair-specific O-methyltransferase designated SbOMT3. Transgenic plants which express SbOMT3 can convert resveratrol into pterostilbene in planta. SbOMT3 is also involved in the biosynthesis of sorgoleone.
Claims28
An isolated or recombinant nucleic acid comprising a sequence that encodes a polypeptide having the amino acid sequence set forth in SEQ ID NO:2.
An isolated or recombinant nucleic acid comprising a sequence that encodes a polypeptide comprising an amino acid sequence having at least 90% sequence identity to the amino acid sequence set forth in SEQ ID NO:2, wherein the polypeptide has O-methyltransferase activity.
An isolated or recombinant nucleic acid comprising a sequence that encodes a polypeptide comprising an amino acid sequence having at least 95% sequence identity to the amino acid sequence set forth in SEQ ID NO:2, wherein the polypeptide has O-methyltransferase activity.
An isolated or recombinant nucleic acid comprising a sequence that encodes a polypeptide comprising an amino acid sequence having at least 99% sequence identity to the amino acid sequence set forth in SEQ ID NO:2, wherein the polypeptide has O-methyltransferase activity.
An isolated or recombinant nucleic acid comprising the sequence set forth in SEQ ID NO:1.
An isolated or recombinant nucleic acid comprising a sequence that encodes a polypeptide having O-methyltransferase activity, said sequence comprising a sequence having at least 90% sequence identity to SEQ ID NO:1, as determined by analysis with a sequence comparison algorithm or by visual inspection, or a sequence fully complementary to said sequence.
An isolated or recombinant nucleic acid comprising a sequence that encodes a polypeptide having O-methyltransferase activity, said sequence comprising a sequence having at least 95% sequence identity to SEQ ID NO:1, as determined by analysis with a sequence comparison algorithm or by visual inspection, or a sequence fully complementary to said sequence.
An isolated or recombinant nucleic acid comprising a sequence that encodes a polypeptide having O-methyltransferase activity, said sequence comprising a sequence having at least 99% sequence identity to SEQ ID NO:1, as determined by analysis with a sequence comparison algorithm or by visual inspection, or a sequence fully complementary to said sequence.
A method of increasing pterostilbene content in a plant or plant cell comprising introducing into a plant at least one DNA construct comprising a nucleotide operably linked to a promoter that drives expression in a plant cell, wherein said nucleotide sequence is selected from the group consisting of:
- (a) an isolated or recombinant nucleic acid comprising a sequence that encodes a polypeptide having the amino acid sequence set forth in SEQ ID NO:2;
- (b) an isolated or recombinant nucleic acid comprising a sequence that encodes a polypeptide comprising an amino acid sequence having at least 90% sequence identity to the amino acid sequence set forth in SEQ ID NO:2, wherein the polypeptide has O-methyltransferase activity;
- (c) an isolated or recombinant nucleic acid comprising a sequence that encodes a polypeptide comprising an amino acid sequence having at least 95% sequence identity to the amino acid sequence set forth in SEQ ID NO:2, wherein the polypeptide has O-methyltransferase activity;
- (d) an isolated or recombinant nucleic acid comprising a sequence that encodes a polypeptide comprising an amino acid sequence having at least 99% sequence identity to the amino acid sequence set forth in SEQ ID NO:2, wherein the polypeptide has O-methyltransferase activity;
- (e) an isolated or recombinant nucleic acid comprising the sequence set forth in SEQ ID NO:1;
- (f) an isolated or recombinant nucleic acid comprising a sequence that encodes a polypeptide having O-methyltransferase activity, said sequence comprising a sequence having at least 90% sequence identity to SEQ ID NO:1, as determined by analysis with a sequence comparison algorithm or by visual inspection;
- (g) an isolated or recombinant nucleic acid comprising a sequence that encodes a polypeptide having O-methyltransferase activity, said sequence comprising a sequence having at least 95% sequence identity to SEQ ID NO:1, as determined by analysis with a sequence comparison algorithm or by visual inspection;
- (h) an isolated or recombinant nucleic acid comprising a sequence that encodes a polypeptide having O-methyltransferase activity, said sequence comprising a sequence having at least 99% sequence identity to SEQ ID NO:1, as determined by analysis with a sequence comparison algorithm or by visual inspection;
- and
- (i) an isolated nucleic acid having the sequence set forth in SEQ ID NO:
- 1;
- and then allowing said nucleic acid sequence to be expressed in said plant or plant cell so as to thereby increase the accumulation of pterostilbene in the plant or plant cell.
Dependencies are inferred from the claim text.