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US 7,732,662 B2Granted
Method for the transformation of plant cell plastids
AssigneeMonsanto Technology LLC
InventorsJeffrey M Staub, Guangning Ye, Debra L. Broyles
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Abstract
Novel compositions and methods useful for genetic engineering of plant cells to provide a method of producing plastid transformed plants are provided in the instant invention. In particular, the present invention provides methods for obtaining plastid transformed plants on medium containing plastid lethal compounds.
Claims12
A method for obtaining a transplastomic plant comprising the steps of:
- a) introducing into a plant cell plastid a first recombinant nucleic acid construct comprising a promoter functional in a plant cell plastid, a nucleic acid sequence encoding a protein providing for tolerance to a plastid lethal compound and a transcriptional termination region functional in a plant cell plastid and a second recombinant nucleic acid construct comprising a promoter functional in a plant cell plastid, a nucleic acid sequence encoding a protein providing for tolerance to a plastid non-lethal compound and a transcriptional termination region functional in a plant cell plastid,
- b) placing said plant cell having said first and second nucleic acid constructs on a first culture medium comprising a plastid non-lethal compound for a period of time sufficient to permit said plant cell to replicate,
- c) placing said plant cell on a second culture medium comprising a plastid lethal compound for a period of time sufficient to select plant cells capable of growing in the presence of said plastid lethal compound, and
- d) regenerating a transplastomic plant from said plant cell.
1 more element
Dependencies are inferred from the claim text.