Injecting Drosophila embryos
AssigneeGenetic Services, Inc.
InventorsSusan B. Zusman, Michael Tworoger
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Abstract
The present invention provides systems that allow reliable multiplexed transformation of Drosophila embryos. The present invention provides methods and reagents that allow preparation of injection-quality nucleic acid samples and that allow simultaneous preparation of multiple such samples. The present invention provides systems for simultaneous processing of multiple injected embryos. The present invention provides methods for transformation of Drosophila embryos involving use of virginator strains that can be used to increase the efficiency of setting up the crosses needed to produce the eggs for the injections and for the crosses needed to screen for transformants.
Claims13
A method comprising steps of:
- obtaining a plurality of different nucleic acid samples for injection by needle into Drosophila embryos;
- and
- injecting at least 8 embryos per minute with a nucleic acid from the nucleic acid samples, such that at least 30% of injected embryos become fertile adults.
Dependencies are inferred from the claim text.