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US 7,732,628 B2Granted
Functional biopolymer modification reagents and uses thereof
AssigneeSoluLink, Inc.
InventorsDavid A. Schwartz, Richard I. Hogrefe
Life of this patent
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Abstract
Hydrazino, oxyamino and carbonyl-based reagents and methods for incorporation into oligonucleotides during their solid phase synthesis are provided. Modified oligonucleotides are provided that incorporate the reagents provided herein. Immobilized oligonucleotides and oligonucleotide conjugates that contain covalent hydrazone or oxime linkages are provided. Methods for preparation of surface bound oligonucleotides are provided. Methods for the preparation of oligonucleotide conjugates are also provided.
Claims11
A compound that has formula (I):
- P1-M-X
- wherein:
- P1 is a phosphorous based coupling group selected from the group consisting of phosphoramidite, H-phosphonate and phosphotriester;
- X is selected from the group consisting of:
- —NHNH—R4, —NHN═C(CH3)2, —(C═O)CH3, —(C═O)H and —ONHR4
- wherein R4 is a protecting group;
- and
- M is a divalent group of the formula —(CH2)n—NH—(C═R1)—R2— wherein R1 is S or O;
- R2 is pyrimidinyl group, a quinolinyl group or a pyrrolyl group;
- and n is 2, 3, 4, 5, 6, 7, 8, 9, or 10.
7 more elements
Dependencies are inferred from the claim text.