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AssigneesSeoulin Bio Science Co., Ltd., Seoul National University Industry Foundation
InventorsBum-Joon Kim, Yoon-Ho Kook, Jeong-Mi Kim
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The present invention relates to a pair of primers specific to mycobacterial species, a polynucleotide of an hsp 65 gene fragment, and a method for the identification of mycobacterial species by using the same. More specifically, the 604-bp hsp 65 gene fragment can be applied to identification methods of mycobacteria such as the comparative sequence analysis method, the probe hybridization method, and PCR-RFLP, which can resolve the problems of a conventional identification method based on bio-chemical characteristics, where the genus mycobacterium covers various species and has a low growth rate, and of the problems of 16s rDNA. Thus, according to the identification method of the present invention, the mycobacterial species can be identified simply, economically, and accurately.
A polynucleotide of an hsp 65 gene fragment of mycobacterial species, wherein the fragment is amplified by using a pair of primers for amplifying the hsp 65 gene fragment of mycobacterial species, one primer consists of the nucleotide sequence of SEQ ID NO:
A polynucleotide selected from the group of polynucleotides consisting of SEQ ID NOs:
A polynucleotide set for the detection or identification of mycobacterial species wherein the set consists of at least two hsp 65 gene fragments selected from the group of polynucleotides consisting of SEQ ID NOs:
A method for the identification of a mycobacterial species comprising the steps of:
A pair of primers for amplifying an hsp 65 (Heat Shock Protein 65) gene fragment of mycobacterial species, wherein one primer consists of the nucleotide sequence of SEQ ID NO:
Dependencies are inferred from the claim text.