High-affinity monoclonal antibodies for botulinum toxin type A
AssigneeThe United States of America as represented by the Secretary of Agriculture
InventorsLarry H Stanker, Luisa W Cheng, Miles C Scotcher
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Abstract
High affinity antibodies for binding epitopes of BoNT/Aand hybridomas that produce such antibodies are described. The antibodies may be used in a kit for detecting BoNT/A in a sample.
Claims10
An isolated and purified monoclonal antibody produced by the continuous hybridoma cell line having deposit accession number ATCC PTA-8336.
An isolated and purified monoclonal antibody produced by the continuous hybridoma cell line having deposit accession number ATCC PTA-8337.
An isolated and purified monoclonal antibody produced by the continuous hybridoma cell line having deposit accession number ATCC PTA-8338.
A kit for detecting BoNT/A in a sample, said kit comprising:
- (1) a container comprising a monoclonal antibody selected from the group consisting of ATCC PTA-8336, ATCC PTA-8337, ATCC PTA-8338, ATCC PTA-8339, and mixtures thereof;
- and (2) instructions for using the antibody for the purpose of binding to BoNT/A to form an immunological complex and detecting the formation of the immunological complex such that presence or absence of immunological complex correlates with presence or absence of BoNT/A in said sample.
A method for detecting BoNT/A comprising (1) incubating a sample with the monoclonal antibody selected from the group consisting of ATTC PTA-8336, ATCC PTA-8337, ATCC PTA-8338, ATCC PTA-8339, and mixtures thereof;
- and (2) detecting the antibody-BoNT/A complex wherein the presence or absence of the complex indicates the presence or absence of BoNT/A in the sample.
Dependencies are inferred from the claim text.