Compositions and methods relating to elutable carbohydrate-binding proteins
AssigneeNew England Biolabs, Inc.
InventorsChristopher H. Taron, Paul A. Colussi, Jeremiah Read
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Abstract
Compositions and methods are provided for creating and identifying mutant carbohydrate-binding proteins that reversibly bind to carbohydrate substrates under conditions where the native protein remains bound. Examples of modified chitin-binding domains are provided which can be eluted from chitin in the presence of a reducing agent or at a pH within the range of 5-10.
Claims4
A chitin-binding domain derived from Kluyveromyces having an amino acid sequence comprising a serine or a threonine at an amino acid position corresponding to a glycine at position 524 in a Kluyveromyces chitinase identified as glycine 55 in SEQ ID. NO:
- 1;
- the chitin-binding domain capable of reversibly binding to chitin.
Dependencies are inferred from the claim text.