Methods and compositions for RNA interference using recombinant Dicer and Argonaut
AssigneeCold Spring Harbor Laboratory
InventorsDavid Beach, Emily Bernstein, Amy Caudy, Scott Hammond, Gregory Hannon
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Abstract
The present invention provides methods for attenuating gene expression in a cell using gene-targeted double stranded RNA (dsRNA). The dsRNA contains a nucleotide sequence that hybridizes under physiologic conditions of the cell to the nucleotide sequence of at least a portion of the gene to be inhibited (the “target” gene).
Claims12
A method for attenuating expression of a target gene in a non-embryonic mammalian cell in culture, comprising introducing into the cell by transfection a double stranded RNA (dsRNA) in an amount sufficient to attenuate expression of the target gene, wherein the dsRNA is about 22 nucleotides in length and complementary across its length to a nucleotide sequences of the target gene and does not activate protein kinase RNA-activated (PKR) sequence-independent response, and wherein the cell is engineered with (i) a recombinant gene encoding a Dicer activity, (ii) a recombinant gene encoding an Argonaut activity, or (iii) both.
Dependencies are inferred from the claim text.