Method of conducting homologous recombination
AssigneeNational University Corporation Saitama University
InventorHirokazu Inoue
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Abstract
The present invention provides a novel method of significantly promoting the ratio of homologous recombination in desirable cells. In order to enhance the ratio of homologous recombination in desirable cells such as eukaryotic cells, mutation is introduced into genes encoding factors necessary for non-homologous recombination, such as Ku70 or Ku80, or the above genes are disrupted, so as to cause the loss of the functions thereof. At the time, foreign DNA is introduced into the cells via the electroshock method or the like, so as to carry out homologous recombination, thereby promoting the frequency of homologous recombination in the cells.
Claims9
A method of conducting homologous recombination, which is characterized in that it comprises the following steps (a) and (b):
- (a) a step of preparing filamentous fungi belonging to genus Neurospora or Aspergillus wherein a decrease or loss of the functions of a gene selected from the group consisting of KU70, KU80, LIGIV and XRCC4 is induced;
- and
- (b) a step of introducing foreign DNA into said filamentous fungi, so as to conduct homologous recombination.
Dependencies are inferred from the claim text.