Anti-angiogenic methods and compositions
AssigneeSangamo BioSciences, Inc.
InventorsH. Steve Zhang, Philip D. Gregory, Edward J. Rebar
Life of this patent
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Abstract
Disclosed herein are methods and compositions for treatment of conditions requiring inhibition of angiogenesis. Such conditions include those characterized by neovascularization, such as retinopathies, macular degeneration and various malignancies.
Claims26
A polynucleotide encoding two engineered zinc finger proteins, wherein the first zinc finger protein comprises six zinc fingers and the amino acid sequence of the recognition region of each of the zinc fingers is as follows:
- F1:
- RSDALSR
- (SEQ ID NO:
- 14)
- F2:
- QSGDLTR
- (SEQ ID NO:
- 15)
- F3:
- QSGDLTR
- (SEQ ID NO:
- 15)
- F4:
- TSGHLSR
- (SEQ ID NO:
- 16)
- F5:
- RSDHLSN
- (SEQ ID NO:
- 17)
- F6:
- QSATRIT;
- (SEQ ID NO:
- 18)
- and
- wherein the second zinc finger protein comprises three zinc fingers and the amino acid sequence of the recognition region of each of the zinc fingers is as follows:
- F1:
- DRSNLTR
- (SEQ ID NO:
- 83)
- F2:
- TSGHLSR
- (SEQ ID NO:
- 16)
- F3:
- RSDHLSR
- (SEQ ID NO:
- 84).
A polynucleotide encoding two engineered zinc finger proteins, wherein the first zinc finger protein comprises six zinc fingers and the amino acid sequence of the recognition region of each of the zinc fingers is as follows:
- F1:
- RSDALSR
- (SEQ ID NO:
- 14)
- F2:
- QSGDLTR
- (SEQ ID NO:
- 15)
- F3:
- QSGDLTR
- (SEQ ID NO:
- 15)
- F4:
- TSGHLSR
- (SEQ ID NO:
- 16)
- F5:
- RSDHLSN
- (SEQ ID NO:
- 17)
- F6:
- QSATRIT;
- (SEQ ID NO:
- 18)
- and
- wherein the second zinc finger protein comprises six zinc fingers and the amino acid sequence of the recognition region of each of the zinc fingers is as follows:
- F1:
- RSDALSE
- (SEQ ID NO:
- 65)
- F2:
- DSSHRTR
- (SEQ ID NO:
- 60)
- F3:
- RSDHLSA
- (SEQ ID NO:
- 61)
- F4:
- ANSNRIK
- (SEQ ID NO:
- 62)
- F5:
- QSSDLSR
- (SEQ ID NO:
- 58)
- F6:
- RSDALAR
- (SEQ ID NO:
- 32).
Dependencies are inferred from the claim text.