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US 7,732,195 B2Granted
Tethered vectors for cell surface immunoglobulin display
AssigneeFacet Biotech Corporation
InventorsYoshiko Akamatsu, Tsuneaki Asai, Naoya Tsurushita
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Abstract
Disclosed are mammalian cell surface display vectors for isolating and/or characterizing immunoglobulins and various uses thereof.
Claims22
A vector for expressing immunoglobulins comprising:
- an origin of replication;
- a first polynucleotide encoding an immunoglobulin heavy chain constant domain or an immunoglobulin light chain constant domain;
- a second polynucleotide encoding an immunoglobulin heavy chain constant domain or an immunoglobulin light chain constant domain;
- and
- a polynucleotide encoding a cell surface tether domain selected from the group consisting of platelet derived growth factor receptor (PDGF-R) transmembrane domain (SEQ ID NO:4), B7-1 (CD80) transmembrane domain (SEQ ID NO:6), and glycosidylphosphatidylinositol (GPI) signal domain (SEQ ID NO:2),
- wherein said polynucleotide encoding a cell surface tether domain is flanked by a first and a second restriction endonuclease recognition site.
3 more elements
A isolated host cell comprising a vector for expressing immunoglobulin comprising:
- an origin of replication;
- a first polynucleotide encoding an immunoglobulin heavy chain constant domain or an immunoglobulin light chain constant domain;
- a second polynucleotide encoding an immunoglobulin heavy chain constant domain or an immunoglobulin light chain constant domain;
- a polynucleotide encoding an immunoglobulin light chain variable domain;
- a polynucleotide encoding an immunoglobulin heavy chain variable domain;
- and
- a polynucleotide encoding a cell surface tether domain selected from the group consisting of platelet derived growth factor receptor (PDGF-R) transmembrane domain (SEQ ID NO:4), B7-1(CD80) transmembrane domain (SEQ ID NO:6), and glycosidylphosphatidylinositol (GPI) signal domain (SEQ ID NO:2);
- wherein said polynucleotide encoding a cell surface tether domain is flanked by a first and a second restriction endonuclease recognition site.
5 more elements
Dependencies are inferred from the claim text.