Characterization of the I-SpomI endonuclease from fission yeast
AssigneesInstitut Pasteur, Centre National De La Recherche Scientifique, Universite Pierre et Marie Curie (Paris VI)
InventorsStefan Pellenz, Bernard Dujon, Alexis Harington, Bernd Schafer
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Abstract
Isolated DNAs encoding the enzyme I-SpomI and its recognition and cutting site are provided. The DNA sequences can be incorporated in cloning and expression vectors, transformed cell lines and transgenic animals. The vectors are useful in gene mapping and site-directed insertion of genes.
Claims20
The plasmid deposited at CNCM under accession number I-2643.
An isolated DNA sequence that encodes a polypeptide having I-SpomI endonuclease activity, wherein said DNA sequence consists of a nucleotide sequence that encodes the amino acid sequence of SEQ ID NO:12.
An expression vector comprising a DNA sequence that encodes a polypeptide having I-SpomI endonuclease activity, wherein said DNA sequence consists of a nucleotide sequence that encodes the amino acid sequence of SEQ ID NO:12, and wherein said expression vector is capable of directing expression of said polypeptide having I-SpomI endonuclease activity.
A recombinant chromosome comprising a DNA sequence that encodes a polypeptide having I-SpomI endonuclease activity, wherein said DNA sequence consists of a nucleotide sequence that encodes the amino acid sequence of SEQ ID NO:12, and wherein said recombinant chromosome is capable of directing expression of said polypeptide having I-SpomI endonuclease activity.
A recombinant cell comprising nucleic acid comprising a DNA sequence that encodes a polypeptide having I-SpomI endonuclease activity, wherein said DNA sequence consists of a nucleotide sequence that encodes the amino acid sequence of SEQ ID NO:12, and wherein said nucleic acid is capable of directing expression of said polypeptide having I-SpomI endonuclease activity.
A non-human transgenic organism comprising a nucleic acid comprising a DNA sequence that encodes a polypeptide having I-Spoml endonuclease activity, wherein said DNA sequence consists of a nucleotide sequence that encodes the amino acid sequence of SEQ ID NO:12, and wherein said nucleic acid is capable of directing expression of said polypeptide having I-Spoml endonuclease activity.
Dependencies are inferred from the claim text.