Method for expression of small RNA molecules within a cell
AssigneeCalifornia Institute of Technology
InventorsCarlos Lois-Caballe, David Baltimore, Xiao-Feng Qin
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Abstract
The invention provides methods and compositions for the expression of small RNA molecules within a cell using a lentiviral vector. The methods can be used to express doubles stranded RNA complexes. Small interfering RNA (siRNA) can be expressed using the methods of the invention within a cell, which are capable of down regulating the expression of a target gene through RNA interference. A variety of cells can be treated according to the methods of the invention including embryos, embryogenic stem cells, allowing for the generation of transgenic animals or animals constituted partly by the transduced cells that have a specific gene or a group of genes down regulated.
Claims62
A method of expressing an siRNA within a cell, the method comprising:
- transfecting a packaging cell line with a retroviral construct;
- recovering recombinant retrovirus from the packaging cell line;
- and
- infecting a target cell in vitro with the recombinant retrovirus,
- wherein the retroviral construct comprises the R and U5 sequences from a 5′ lentiviral long terminal repeat (LTR), a self-inactivating lentiviral 3′ LTR, an RNA Polymerase III promoter region and an RNA coding region encoding an siRNA operably linked to an RNA Polymerase III promoter region;
- wherein the RNA polymerase III promoter region and the RNA coding region are located between the 5′ LTR and the 3′ LTR;
- wherein the RNA coding region encodes a self-complementary RNA molecule having a sense region, an antisense region and a loop region;
- and
- wherein the sense region and the antisense region are each between about 15 and about 30 nucleotides in length.
A retroviral construct for the expression of an siRNA within a cell, the retroviral construct comprising:
- a nucleic acid having the R and U5 sequences from a 5′ lentiviral long terminal repeat (LTR);
- a self-inactivating lentiviral 3′ LTR;
- a first RNA Polymerase III promoter, and
- at least a first RNA coding region encoding a first siRNA operably linked to the first RNA Polymerase III promoter;
- wherein the first RNA polymerase III promoter and the first RNA coding region are located between the 5′ LTR and the 3′ LTR.
A method of expressing an siRNA within a cell, the method comprising:
- infecting a target cell in vitro with a recombinant retrovirus comprising the R and U5 sequences from a 5′ lentiviral long terminal repeat (LTR), a self-inactivating lentiviral 3′ LTR, an RNA Polymerase III promoter region, and an RNA coding region encoding an siRNA operably linked to the RNA Polymerase III promoter region,
- wherein the RNA polymerase III promoter region and the RNA coding region are located between the 5′ LTR and the 3′ LTR.
A method of expressing an siRNA within a cell, the method comprising:
- transfecting a packaging cell line with a retroviral construct;
- recovering recombinant retrovirus from the packaging cell line;
- and infecting a target cell in vitro with the recombinant retrovirus, wherein the retroviral construct comprises the R and U5 sequences from a 5′ lentiviral long terminal repeat (LTR), a self-inactivating lentiviral 3′ LTR, a first RNA coding region encoding a first siRNA strand operably linked to a first RNA polymerase III promoter, and a second RNA coding region encoding a second siRNA strand operably linked to a second RNA polymerase III promoter, wherein the second siRNA strand is substantially complementary to the first siRNA strand, wherein the first and second RNA polymerase III promoters and first and second RNA coding regions are located between the 5′ LTR and the 3′ LTR.
A method of expressing an siRNA within a cell, the method comprising:
- transfecting a packaging cell line with a retroviral construct;
- recovering recombinant retrovirus from the packaging cell line;
- and infecting a target cell in vitro with the recombinant retrovirus, wherein the retroviral construct comprises the R and U5 sequences from a 5′ lentiviral long terminal repeat (LTR), a self-inactivating lentiviral 3′ LTR, an siRNA coding region, a first RNA polymerase III promoter, and a second RNA polymerase III promoter, wherein the first and second RNA polymerase III promoters are operably linked to the siRNA coding region such that expression of the siRNA coding region from the first RNA polymerase III promoter results in the synthesis of a first siRNA and expression of the siRNA coding region from the second RNA polymerase III promoter results in the synthesis of a second siRNA, wherein the first and second RNA polymerase III promoters and the siRNA coding regions are located between the 5′ LTR and the 3′ LTR.
Dependencies are inferred from the claim text.