Modified two-component gelation systems, methods of use and methods of manufacture

A kit for a post-myocardial infarct bioscaffolding gel, the kit separately comprising the following components:

• (a) a first mixture comprising a first functionalized polymer in a first buffer, the first buffer having a physiological osmolality;
• (b) a second buffer having a concentration of 140 mM to 150 mM and a pH between 7.5 and 9;
• (c) a second functionalized polymer different from the first functionalized polymer, the second functionalized polymer capable of being combined with the first mixture or the second buffer;
• and
• (d) a proteinaceous or peptide substance having at least one cell-adhesion site, the substance capable of being combined with the first mixture,
• wherein the first functionalized polymer is a functionalized polyethylene glycol selected from the group consisting of an activated ester-terminated polyethylene glycol or a vinyl-terminated polyethylene glycol;
• wherein the second functionalized polymer is selected from the group consisting of a thiol-terminated polyethylene glycol or an amino-terminated polyethylene glycol;
• and wherein the components, when combined, form a gel having a pH of 7.2.