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US 7,732,185 B2Granted
Plasmid having response to dioxins, transgenic cell for measuring dioxins, dioxins sensing method and biosensor using the same
AssigneeYamanashi University
InventorsMasanori Kitamura, Shuichiro Maeda
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Abstract
A plasmid comprising a gene, which is reactive with dioxins and/or polycyclic aromatic hydrocarbons to thereby be activated (hereinafter referred to as DRE gene), and a secretory marker protein expressing gene disposed understream of the DRE gene. Further, there is developed a transgenic cell having this plasmid introduced therein which when exposed to dioxins and/or polycyclic aromatic hydrocarbons, secretes a secretory marker protein. Still further, there is developed a biosensor utilizing this transgenic cell.
Claims6
A transgenic cell comprising a plasmid comprising,
- a dioxin responsive enhancer (DRE) gene sequence, wherein the DRE gene sequence is integrated into a mouse mammary tumor virus (MMTV) promoter to obtain a MMTV-DRE promoter sequence and
- a secreted alkaline phosphatase protein expressing gene disposed downstream of the MMTV-DRE promoter sequence
- wherein the MMTV-DRE promoter sequence is inducible upon reaction with dioxins and/or polycyclic aromatic hydrocarbons and
- wherein the cell is a Hepa-1c1c7 cell.
1 more element
Dependencies are inferred from the claim text.