Biomolecule partition motifs and uses thereof
AssigneeThe Johns Hopkins University
InventorsMin Li, Sojin Shikano
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Abstract
The invention provides amino acid sequence motifs (e.g., biomolecule partition motifs) that can direct targeting of intracellular polypeptides to and through membranes, including cell surface membranes.
Claims12
A recombinant polynucleotide that encodes a cell surface polypeptide comprising a biomolecule partition motif, wherein the biomolecule partition motif comprises the amino acid sequence of SEQ ID NO:
- 250 and is located at the carboxy terminus of said polypeptide.
An expression vector comprising a recombinant polynucleotide that encodes a cell surface polypeptide comprising a biomolecule partition motif, wherein the biomolecule partition motif comprises the amino acid sequence of SEQ ID NO:
- 250 and is located at the carboxy terminus of said polypeptide.
A process for preparing a transformed cell, the process comprising the steps of:
- a) transfecting, electroporating or transforming the cell with a recombinant polynucleotide that encodes a cell surface polypeptide comprising a biomolecule partition motif, wherein the biomolecule partition motif comprises the amino acid sequence of SEQ ID NO:
- 250 and is located at the carboxy terminus of said polypeptide;
- and
- b) maintaining the cell of step a) under conditions sufficient for expression of the polypeptide of step a).
A transformed cell comprising a recombinant cell surface polypeptide, wherein the recombinant cell surface polypeptide comprises a biomolecule partition motif, wherein the biomolecule partition motif comprises the amino acid sequence of SEQ ID NO:
- 250 and is located at the carboxy terminus of said polypeptide, and wherein the biomolecule partition motif is not present in the native polypeptide.
A method of obtaining a polypeptide from the surface of a cell, the method comprising:
- expressing a polypeptide having at least one biomolecule partition motif in a cell under conditions sufficient to provide the polypeptide to the surface of the cell, wherein the biomolecule partition motif comprises the amino acid sequence of SEQ ID NO:
- 250 and is located at the carboxy terminus of said polypeptide;
- and
- isolating the polypeptide,
- thereby obtaining the polypeptide from the surface of the cell.
Dependencies are inferred from the claim text.