Method for producing α-1, 6-branched α-1, 4-glucans from sucrose
AssigneeBayer BioScience N.V.
InventorsVolker Büttcher, Martin Quanz
Life of this patent
Estimating the term…
Abstract
Nucleic acid molecules which encode a branching enzyme from a bacterium of the genus Neisseria, vectors, host cell, plant cells and plants containing said nucleic acid molecules as well as starch obtainable from the plants described are described. Furthermore, an in-vitro method for producing α-1,6-branched α-1,4-glucans on the basis of sucrose and a combination of enzymes of an amylosucrase and a branching enzyme as well as the α-1,6-branched α-1,4-glucans obtainable by said method are described.
Claims48
An isolated nucleic acid molecule encoding a branching enzyme selected from the group consisting of
- (a) a nucleic acid molecule encoding a protein which comprises the amino acid sequence depicted in SEQ ID NO:2;
- (b) a nucleic acid molecule comprising the coding region depicted in SEQ ID NO:1;
- (c) a nucleic acid molecule encoding a protein which comprises the amino acid sequence encoded by the insert in plasmid DSM 12425;
- (d) a nucleic acid molecule comprising the coding region which is contained in the insert of the plasmid DSM 12425;
- And
- (e) a nucleic acid molecule having more than 95% identity with SEQ ID NO:1.
A method for producing a branching enzyme from a bacterium of the genus Neisseria, wherein a host cell which is genetically modified with a vector containing a nucleic acid molecule selected from the group consisting of
- (a) nucleic acid having more than 95% identity with SEQ ID NO:1;
- (b) nucleic acid which encodes the amino acid sequence depicted in SEQ ID NO:2;
- (c) nucleic acid molecule which has the sequence depicted in SEQ ID NO:1;
- and
- (d) nucleic acid molecule which encodes the amino acid sequence encoded by the insert in plasmid DSM 12425;
- is cultivated under conditions allowing the expression of the enzyme, and wherein the enzyme is isolated from the cultivated cells and/or the culture medium.
A host cell which is genetically modified with a vector containing a nucleic acid molecule selected from the group consisting of
- (a) nucleic acid having more than 95% identity with SEQ ID NO:1;
- (b) nucleic acid which encodes the amino acid sequence depicted in SEQ ID NO:2;
- (c) a nucleic acid molecule which has the sequence depicted in SEQ ID NO:1;
- and
- (d) nucleic acid molecule which encodes the amino acid sequence encoded by the insert in plasmid DSM 12425;
- wherein the host cell is an E. coli cell.
A host cell which is genetically modified with a vector containing a nucleic acid molecule selected from the group consisting of
- (a) nucleic acid having more than 95% identity with SEQ ID NO:1;
- (b) nucleic acid which encodes the amino acid sequence depicted in SEQ ID NO:2;
- (c) nucleic acid molecule which has the sequence depicted in SEQ ID NO:1;
- and
- (d) nucleic acid molecule which encodes the amino acid sequence encoded by the insert in plasmid DSM 12425;
- wherein the host cell is a fungal cell.
Dependencies are inferred from the claim text.