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US 7,732,161 B2Granted
Production of isoprenoids
AssigneeBio-Technical Resources, a division of Arkion Life Sciences, LLC
InventorsJames R. Millis, Julie Maurina-Brunker, Thomas W. McMullin
Life of this patent
Estimating the term…
Abstract
The invention provides a biological method of producing isoprenoids.
Claims37
A fermentation method comprising:
- a. contacting a recombinant yeast with a fermentation medium wherein the recombinant yeast comprises a plurality of chromosomally integrated recombinant nucleic acid molecules, wherein each recombinant nucleic acid molecule encodes an enzyme that converts hydroxymethylglutaryl-CoA to L-mevalonic acid;
- wherein the recombinant yeast is capable of producing an isoprenoid product having 5, 10, 15 or 20 carbons and wherein the fermentation medium comprises a sugar carbon source in a first amount between about 1 gram per liter and about 100 gram per liter of fermentation medium;
- b. culturing the recombinant yeast under conditions wherein the sugar carbon source in the fermentation medium is consumed such that the fermentation medium comprises the sugar carbon source in a second amount which is less than the first amount;
- c. maintaining the culturing conditions such that the fermentation medium comprises the sugar carbon source in an amount that is equal to or less than the second amount;
- and
- d. recovering the product.
3 more elements
A fermentation method, comprising:
- a. culturing a recombinant yeast that is capable of producing an isoprenoid product having 5, 10, 15 or 20 carbons, comprising a plurality of chromosomally integrated recombinant nucleic acid molecules, wherein each recombinant nucleic acid molecule encodes an enzyme that converts hydroxymethylglutaryl-CoA to L-mevalonic acid in a fermentation medium under fed-batch conditions until the microorganism reaches a cell density of at least 10 grams of dry cell weight per liter of fermentation medium;
- and
- b. recovering the isoprenoid product.
Dependencies are inferred from the claim text.