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US 7,732,160 B2Granted
Methamphetamine derivatives and conjugates for immunoassay
AssigneeRoche Diagnostics Operations, Inc.
InventorsRaymond A. Hui, Stephen S. Vitone
Life of this patent
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Abstract
Compounds of methamphetamine derivatives having a meta-substituted alkyl linker on the benzene ring and a protective group on the nitrogen of the methamphetamine hapten. Such compounds have the structure. wherein R1 is an alkyl linker comprising 2-15 carbon atoms and 0-6 heteroatoms, R2 is a leaving group, and R3 is a protecting group.
Claims4
An immunoassay for determining methamphetamine in a sample comprising the steps of:
- combining a sample suspected of containing methamphetamine with an antibody specific for methamphetamine and a labeled analyte analog having the structure
- wherein R1 is an alkyl linker comprising 2-15 carbon atoms and 0-6 heteroatoms and R2 is aminodextran, wherein the antibody is conjugated to a microparticle, whereby the methamphetamine and the analyte analog competitively bind to the antibody, and
- determining the amount of labeled analog bound or unbound to the antibody as a measure of the methamphetamine in the sample.
A test kit for use in determining methamphetamine in a sample comprising:
- an antibody specific for methamphetamine and a labeled analyte analog having the structure
- wherein R1 is an alkyl linker comprising 2-15 carbon atoms and 0-6 heteroatoms and R2 is aminodextran, wherein the antibody is conjugated to a microparticle.
Dependencies are inferred from the claim text.