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US 7,732,155 B2Granted
Methods for identifying lysophosphatidylcholine acyltransferases
AssigneesNational Research Council of Canada, Dow Agrosciences LCC
InventorsJitao Zou, Qilin Chen, Zhifu Zheng, Jingyu Xu
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Abstract
Nucleic acid molecules identified in encode lyso-phosphatidylcholine (LPC) acyltransferases. Over-expression of the LPC acyltransferases in a cell may lead to enhanced production of PUFA, or other unusual fatty acids, and/or to increased oil content in the cell.
Claims12
A method for identifying a lyso-phosphatidylcholine acyltransferase, the method comprising:
- screening a peptide for SEQ ID NO:49;
- wherein Xaa at position 3 of SEQ ID NO:
- 49 is selected from the group consisting of phenylalanine, methionine, valine, and isoleucine;
- wherein Xaa at position 7 of SEQ ID NO:
- 49 is selected from the group consisting of phenylalanine, valine, threonine, and leucine;
- wherein Xaa at position 8 of SEQ ID NO:
- 49 is selected from the group consisting of tyrosine, serine, and arginine;
- wherein Xaa at position 12 of SEQ ID NO:
- 49 is selected from the group consisting of tyrosine, methionine, and isoleucine;
- wherein Xaa at position 13 of SEQ ID NO:
- 49 is selected from the group consisting of leucine, methionine, isoleucine, and phenylalanine;
- and
- wherein Xaa at position 14 of SEQ ID NO:
- 49 is selected from the group consisting of threonine and phenylalanine;
- and
- testing said screened peptide comprising SEQ ID NO:49 for lyso-phosphatidylcholine acyltransferase function, so as to identify the lyso-phosphatidylcholine acyltransferase.
13 more elements
A method for identifying a lyso-phosphatidylcholine acyltransferase, the method comprising:
- screening a peptide for SEQ ID NO:49,
- wherein Xaa at position 3 of SEQ ID NO:
- 49 is selected from the group consisting of phenylalanine, methionine, valine, and isoleucine;
- wherein Xaa at position 7 of SEQ ID NO:
- 49 is selected from the group consisting of phenylalanine, valine, threonine, and leucine;
- wherein Xaa at position 8 of SEQ ID NO:
- 49 is selected from the group consisting of tyrosine, serine, and arginine;
- wherein Xaa at position 12 of SEQ ID NO:
- 49 is selected from the group consisting of tyrosine, methionine, and isoleucine;
- wherein Xaa at position 13 of SEQ ID NO:
- 49 is selected from the group consisting of leucine, methionine, isoleucine, and phenylalanine;
- wherein Xaa at position 14 of SEQ ID NO:
- 49 is selected from the group consisting of threonine and phenylalanine;
- and
- testing a screened peptide comprising SEQ ID NO:49 for lyso-phosphatidylcholine acyltransferase function, wherein said lyso-phosphatidylcholine acyltransferase function may be determined by an assay comprising:
- interacting the screened peptide comprising SEQ ID NO:49 with 14C-labelled lyso-phosphatidylcholine as an acceptor and different unlabeled acyl-CoA as an acyl donor to determine if acylation of lyso-phosphatidylcholine to form phosphatidylcholine is catalyzed by the screened peptide comprising SEQ ID NO:49, so as to identify the screened peptide as a lyso-phosphatidylcholine acyltransferase.
13 more elements
Dependencies are inferred from the claim text.