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US 7,732,151 B2Granted
Use of IRS-polypeptides for identifying of pharmaceutically active compounds
AssigneeSanofi-Aventis Deutschland GmbH
InventorsNorbert Tennagels, Aimo Kannt, Harald Thuering
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Abstract
An assay for determining the ability of an enzyme, functional fragment, or functional derivative thereof to modify the phosphorylation status of a biotinylated polypeptide.
Claims15
A method for determining the ability of a first polypeptide to modify the phosphorylation status of a second, biotinylated polypeptide, the method comprising the steps of
- a. contacting the first polypeptide with the second polypeptide in a suitable reaction mixture;
- b. contacting the reaction mixture with a means which is coupled to a carrier and is able to bind to the second polypeptide;
- and
- c. determining the phosphorylation state of the second polypeptide,
- wherein the carrier comprises a first carrier and a second carrier, wherein the first carrier comprises a first signal generator and the second carrier comprises a second signal generator, wherein the first carrier is coupled to the means and the second carrier is coupled to the second polypeptide.
2 more elements
Dependencies are inferred from the claim text.