Methods of screening agonistic antibodies
AssigneeChugai Seiyaku Kabushiki Kaisha
InventorsTetsuo Kojima, Chiaki Senoo
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Abstract
Cell strains with ligand-dependent (factor-dependent) proliferation are super-infected with antibody libraries against each of the various receptor chains. Antibody genes are recovered from strains that autonomously grow by the autocrine stimulation by agonistic antibodies formed by appropriate combinations, enabling effective screening of agonistic antibodies. In this method, effective screening can be carried out using antibodies as libraries. Furthermore, the manipulations are simple, and there is no need for complicated operations.
Claims15
A method of screening for agonistic antibodies, the method comprising:
- (a) providing a cell that expresses both a multimer-forming receptor and a test antibody, wherein the cell in the absence of the antibody requires a ligand of the receptor for growth;
- (b) culturing the cell in the absence of the ligand;
- and
- (c) selecting the test antibody as an agonist of the receptor if the cell grows in the absence of the ligand.
A method of screening for agonistic antibodies, the method comprising:
- providing an antibody expression library of cells, the cells each expressing both a member of a set of diverse antibodies and a multimer-forming receptor, wherein the cells in the absence of the antibodies require a ligand of the receptor for cell growth;
- culturing the library of cells in the absence of the ligand;
- selecting a cell that grows in the absence of the ligand;
- and
- identifying the antibody expressed by the selected cell as being an agonist of the receptor.
Dependencies are inferred from the claim text.