US 7,732,146 B2Granted

Method for screening an agent that modulates activity of macrophage migration inhibitory factor

AssigneeAvanir Pharmaceuticals

InventorsFederico C. A. Gaeta, Andrew Baird, Jerry Anchin, Wenbin Ying, Robert Florkiewicz, Jagadish Sircar, Sunil Kumar K.C.

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Abstract

Inhibitors of MIF are provided which have utility in the treatment of a variety of disorders, including the treatment of pathological conditions associated with MIF activity. The inhibitors of MIF have the following structures: including stereoisomers, prodrugs and pharmaceutically acceptable salts thereof, wherein n, R1, R2, R3, R4, X, Y and Z are as defined herein. Compositions containing an inhibitor of MIF in combination with a pharmaceutically acceptable carrier are also provided, as well as methods for use of the same.

Claims9

Dependent claims

A method for determining whether an agent inhibits the binding of macrophage migration inhibitory factor to an anti-macrophage migration inhibitory factor monoclonal antibody comprising:

contacting macrophage migration inhibitory factor produced by THP-1 cells with an agent and thereafter with an anti-macrophage migration inhibitory factor monoclonal antibody, and thereafter measuring a first amount of macrophage migration inhibitory factor bound to the anti-macrophage migration inhibitory factor monoclonal antibody;
contacting macrophage migration inhibitory factor produced by THP-1 cells with the anti-macrophage migration inhibitory factor monoclonal antibody in the absence of the agent, and thereafter measuring a second amount of macrophage migration inhibitory factor bound to the anti-macrophage migration inhibitory factor monoclonal antibody;
and
comparing the first amount of bound macrophage migration inhibitory factor in the presence of the agent with the second amount of bound macrophage migration inhibitory factor in the absence of the agent, wherein a statistically significant decrease in the first amount of bound macrophage migration inhibitory factor compared to the second amount of bound macrophage migration inhibitory factor is indicative of the agent inhibiting the binding of macrophage migration inhibitory factor to the anti-macrophage migration inhibitory factor monoclonal antibody.

1 more element

Dependencies are inferred from the claim text.

Facts

Application: 11/182,241Filed 15 Jul 2005 · division
Priority: 24 May 2001
Published: US 2005/0282236 A122 Dec 2005
Granted: 8 Jun 2010
Term ends: Not estimatedThe source records don’t support an estimate.

Classification21 codes

C07D 401/12
Containing two hetero rings: linked by a chain containing hetero atoms as chain links
A61P 1/04
Drugs for disorders of the alimentary tract or the digestive system: for ulcers, gastritis or reflux esophagitis, e.g. antacids, inhibitors of…
A61P 3/10
For glucose homeostasis: for hyperglycaemia, e.g. antidiabetics
A61P 5/08
Of the anterior pituitary hormones, e.g. TSH, ACTH, FSH, LH, PRL, GH: for decreasing, blocking or antagonising the activity of the anterior…

Family

Application60/293,642Us provisional application · filed 24 May 2001
Application10/156,650Parent of this division · filed 24 May 2002
US 2005/0282236 A1Pre-grant publication · published 22 Dec 2005
US 7,105,519 B2Parent of this division
US 2003/0195194 A1Published application
US 7,732,146 B2This patent

From the US filing history. Foreign family members aren’t listed.