Method and devices for rapid diagnosis of foot-and-mouth disease

A single step test device for simultaneously detecting and differentiating between a Foot-and-Mouth Disease Virus (FMDV) infection and FMDV vaccination in a test sample, comprising, a membrane strip, said membrane strip comprising an application point for a fluid test sample comprising a labeled binding partner and a labeled control reagent, wherein said fluid test sample moves from a proximal end to a distal end by capillary action, and wherein said distal end of said strip contains a zone having at least one immobilized capture reagent capable of selectively binding an antibody specific for an FMDV structural protein, said FMDV structural protein comprising at least one of VP1, VP2, VP3 or VP4, and a second zone having at least one immobilized capture reagent capable of selectively binding an antibody specific for an FMDV non-structural protein, said FMDV non-structural protein comprising at least one of 3D, 2C or 3ABC, and a third zone of immobilized control capture reagent, and wherein said labeled binding partner is capable of forming a complex with an analyte in said fluid test sample forming a specific binding complex and wherein said capture reagent capable of selectively binding the antibody specific for the FMDV structural protein and/or said capture reagent capable of selectively binding the antibody specific for the FMDV non-structural protein is capable of binding said specific binding complex, wherein the device distinguishes infection from vaccination in a bovine test sample between about 6 to 8 days post infection with at least a 98% accuracy.