Device for thermo-dependent chain reaction amplification of target nucleic acid sequences, measured in real-time
AssigneePall Genesystems
InventorGabriel Festoc
Life of this patent
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Abstract
The present invention concerns a device for amplifying target nucleic acids, reaction cartridges for use in the device, and modes of use of the device.
Claims28
A method for amplifying a nucleic acid using a device comprising at least one circular cartridge having a plurality of reaction chambers and a reservoir, said reaction chambers being connected to the reservoir via channels, at least one heating plate having at least two distinct zones that can be heated to at least two different temperatures, wherein said distinct zones are sections of a disk or sections of a ring, and a means for relative rotational displacement between said at least one circular cartridge and said at least one heating plate, allowing a cyclic variation of the temperature of the reaction chambers wherein said means for relative rotational displacement is connected to said at least one circular cartridge for displacing the cartridge in relative rotation, said method comprising:
- a) at least partially filling said reservoir with a fluid containing a sample of nucleic acids to be analyzed and reagents for carrying out an amplification reaction, with the exception of primers,
- b) distributing said fluid to the reaction chambers of said at least one circular cartridge, in which are located the primers,
- c) employing said means for relative rotational displacement to rotate said at least one circular cartridge to successively bring the contents of each reaction chamber to the at least two temperatures defined by the at least two distinct zones of said at least one heating plate,
- wherein said nucleic acid is amplified.
A method for amplifying a nucleic acid using a device comprising at least one circular cartridge having a plurality of reaction chambers and a reservoir, said reaction chambers being connected to the reservoir via channels, at least one heating plate having at least two distinct zones that can be heated to at least two different temperatures, wherein said distinct zones are sections of a disk or sections of a ring, and a means for relative rotational displacement between said at least one circular cartridge and said at least one heating plate, allowing a cyclic variation of the temperature of the reaction chambers wherein said means for relative rotational displacement is connected to said at least one circular cartridge for displacing the cartridge in relative rotation, said method comprising:
- a) at least partially filling said reservoir with a fluid containing a sample of nucleic acids to be analyzed;
- b) distributing said fluid to the reaction chambers of said at least one circular cartridge, in which are located the primer and the reagents for carrying out an amplification reaction,
- c) employing said means for relative rotational displacement to rotate said at least one circular cartridge to successively bring the contents of each reaction chamber to the at least two temperatures defined by the at least two distinct zones of said at least one heating plate wherein said means for relative rotational displacement comprises displacing said at least one circular cartridge while retaining in place said at least one heating plate,
- wherein said nucleic acid is amplified.
A method for amplifying a nucleic acid using a device comprising at least one circular cartridge having a plurality of reaction chambers and a reservoir, said reaction chambers being connected to the reservoir via channels, at least one heating plate having at least two distinct zones that can be heated to at least two different temperatures, wherein said distinct zones are sections of a disk or sections of a ring, and a means for relative rotational displacement between said at least one circular cartridge and said at least one heating plate, allowing a cyclic variation of the temperature of the reaction chambers wherein said means for relative rotational displacement is connected to said at least one circular cartridge for displacing the cartridge in relative rotation, said method comprising:
- a) at least partially filling said reservoir with a fluid containing a sample of nucleic acids to be analyzed and reagents for carrying out an amplification reaction, with the exception of primers,
- b) distributing said fluid to the reaction chambers of said at least one circular cartridge, in which are located the primers,
- c) employing said means for relative rotational displacement to rotate said at least one circular cartridge to successively bring the contents of each reaction chamber to the at least two temperatures defined by the at least two distinct zones of said at least one heating plate wherein said means for relative rotational displacement comprises displacing said at least one circular cartridge while retaining in place said at least one heating plate,
- wherein said nucleic acid is amplified.
A method for amplifying a nucleic acid using a device comprising at least one circular cartridge having a plurality of reaction chambers and a reservoir, said reaction chambers being connected to the reservoir via channels, at least one heating plate having at least two distinct zones that can be heated to at least two different temperatures, wherein said distinct zones are sections of a disk or sections of a ring, and a means for relative rotational displacement between said at least one circular cartridge and said at least one heating plate, allowing a cyclic variation of the temperature of the reaction chambers wherein said means for relative rotational displacement is a micromotor and a driver or an axle that is moved by means of the micromotor and the driver or axle is connected to said at least one circular cartridge for displacing the cartridge in relative rotation, said method comprising:
- a) at least partially filling said reservoir with a fluid containing a sample of nucleic acids to be analyzed and reagents for carrying out an amplification reaction, with the exception of primers,
- b) distributing said fluid to the reaction chambers of said at least one circular cartridge, in which are located the primers,
- c) employing said means for relative rotational displacement to rotate said at least one circular cartridge to successively bring the contents of each reaction chamber to the at least two temperatures defined by the at least two distinct zones of said at least one heating,
- wherein said nucleic acid is amplified.
A method for amplifying a nucleic acid using a device comprising a circular cartridge having a plurality of reaction chambers, and a reservoir, the reaction chambers being connected to the reservoir via channels, wherein at least a portion of the reaction chambers comprise primers;
- and a heating plate having at least two distinct zones that can be heated to at least two different temperatures, the method comprising:
- a) at least partially filling the reservoir with a fluid containing a sample of nucleic acids to be analyzed and reagents for carrying out an amplification reaction, with the exception of primers,
- b) distributing the fluid to the reaction chambers comprising the primers therein,
- c) rotating the circular cartridge to successively bring the contents of each reaction chamber to the at least two temperatures defined by the at least two distinct zones of the heating plate,
- wherein the nucleic acid is amplified.
A method for amplifying a nucleic acid using a device comprising a circular cartridge having a plurality of reaction chambers and a reservoir, the reaction chambers being connected to the reservoir via channels, wherein at least a portion of the reaction chambers comprise primers and reagents for carrying out an amplification reaction;
- and a heating plate having at least two distinct zones that can be heated to at least two different temperatures, the method comprising:
- a) at least partially filling the reservoir with a fluid containing a sample of nucleic acids to be analyzed;
- b) distributing the fluid to the reaction chambers comprising the primer and reagents therein,
- c) rotating the circular cartridge to successively bring the contents of each reaction chamber to the at least two temperatures defined by the at least two distinct zones of the heating plate, while retaining in place the at least one heating plate,
- wherein the nucleic acid is amplified.
A method for amplifying a nucleic acid using a device comprising a rotatable circular cartridge having a plurality of reaction chambers and a reservoir, the reaction chambers being connected to the reservoir via channels, wherein at least a portion of the reaction chambers comprise primers;
- and a heating plate having at least two distinct zones that can be heated to at least two different temperatures, the method comprising:
- a) at least partially filling the reservoir with a fluid containing a sample of nucleic acids to be analyzed and reagents for carrying out an amplification reaction, with the exception of primers;
- b) distributing the fluid to the reaction chambers of the rotatable circular cartridge comprising the primers therein, wherein the rotatable circular cartridge is connected to a driver or axle, the driver or axle being movable by a micromotor;
- c) moving, by a micromotor, the driver or axle connected to the rotatable circular cartridge, and rotating the rotatable circular cartridge to successively bring the contents of each reaction chamber to the at least two temperatures defined by the at least two distinct zones of the heating plate,
- wherein the nucleic acid is amplified.
A method for amplifying a nucleic acid using a device comprising a rotatable circular cartridge having a plurality of reaction chambers and a reservoir, the reaction chambers being connected to the reservoir via channels, wherein at least a portion of the reaction chambers comprise primers and reagents for carrying out an amplification reaction;
- and a heating plate having at least two distinct zones that can be heated to at least two different temperatures, the method comprising:
- a) at least partially filling the reservoir with a fluid containing a sample of nucleic acids to be analyzed;
- b) distributing the fluid to the reaction chambers of the rotatable circular cartridge comprising the primers and the reagents therein, wherein the rotatable circular cartridge is connected to a driver or axle, the driver or axle being movable by a micromotor;
- c) moving, by a micromotor, the driver or axle connected to the rotatable circular cartridge, and rotating the rotatable circular cartridge to successively bring the contents of each reaction chamber to the at least two temperatures defined by the at least two distinct zones of the heating plate,
- wherein the nucleic acid is amplified.
Dependencies are inferred from the claim text.