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US 7,732,128 B2Granted
Kits for detection of ATP
AssigneePromega Corporation
InventorsKeith Wood, Rita Hannah, Richard A. Moravec
Life of this patent
Estimating the term…
Abstract
Methods and kits for detecting the presence of ATP, for measuring ATP concentrations, and for detecting viable cells using a composition comprising an ATP-dependent enzyme and one or more ATPase inhibitors.
Claims14
A kit for detecting ATP comprising:
- a luciferase component comprising a chemostable variant of a beetle luciferase having at least 95% amino acid sequence identity to any of SEQ ID NOS:
- 1, 2, 3, or 4;
- and
- an ATPase inhibitor component comprising one or more ATPase inhibitors,
- the chemostable variant retaining greater luminescence activity measured by luminescence after contact with the ATPase inhibitor for at least one hour compared to the wild type beetle luciferase.
2 more elements
A kit for detecting ATP in a sample, the kit comprising:
- a luciferase component comprising a chemostable luciferase, the chemostable luciferase selected from a panel of luciferase variants having at least 95% amino acid sequence identity to a wild-type luciferase;
- and
- an ATPase inhibitor component comprising one or more ATPase inhibitors selected from one or more detergents,
- where combination of the luciferase component and the ATPase component forms a reagent composition comprising the chemostable luciferase and the one or more detergents, with the chemostable luciferase having a rate of decline in enzymatic activity after contact with the ATPase inhibitor in the reagent composition measured by a luminescence characterized by a first half life that is greater than a second half life measured for the wild-type luciferase in the ATPase inhibitor.
1 more element
A kit for detecting ATP, the kit comprising:
- a luciferase component comprising a chemostable variant having at least 90% amino acid sequence identity to P. pennsylvanica beetle luciferase (LucPe2);
- and
- an ATPase inhibitor component comprising one or more ATPase inhibitors selected from one or more detergents;
- the chemostable variant retaining greater enzymatic activity after contact with the ATPase inhibitor compared to the wild type beetle luciferase, the enzymatic activity after contact with the ATPase inhibitor measured by luminescence characterized by a first half life that is greater than a second half life measured for the wild-type bettle luciferase in the ATPase inhibitor.
1 more element
Dependencies are inferred from the claim text.