Back to results
US 7,731,969 B1Granted
Methods for developing and producing antigen-specific antibody-producing cells
AssigneeNeoClone Biotechnology International, LLC
InventorsWard C. Tucker, Mark W. Jackson, Rachel H. Kravitz
Life of this patent
Estimating the term…
Abstract
Disclosed are methods for producing immortalized antigen-specific plasma cells and antibodies which include depleting an immunized cell population of CD138-positive cells and activating the depleted cells. The methods may be used to improve the efficiency of obtaining immortalized antigen-specific plasma cells.
Claims24
A method for producing immortalized antigen-specific plasma cells, comprising:
- a) immunizing an animal and isolating splenocytes from the animal;
- b) depleting the isolated splenocytes of CD138-positive cells to obtain depleted immunized cells;
- c) contacting the depleted immunized cells with an activating agent to obtain activated, antigen-specific B-lymphocytes;
- and
- d) immortalizing the activated, antigen-specific B-lymphocytes, thereby producing the immortalized antigen-specific plasma cells.
2 more elements
A method comprising:
- a) immunizing an animal and isolating splenocytes from the animal;
- b) depleting the isolated splenocytes of CD138-positive cells to obtain depleted immunized cells;
- c) contacting the depleted immunized cells with an activating agent to obtain activated, antigen-specific B-lymphocytes;
- and
- d) immortalizing the activated, antigen-specific B-lymphocytes.
2 more elements
Dependencies are inferred from the claim text.