US 7,731,937 B2 · Diagnostic method for paratuberculosis

Abstract

The present invention is intended to provide a diagnostic method for paratuberculosis by which an animal infected with Mycobacterium avium subsp. Paratuberculosis can be diagnosed at a high sensitivity in the sub-clinical infection stage before the specific antibody level begins to increase and a large number of specimens can be treated. The present invention provides: a diagnostic method for paratuberculosis characterized by collecting the blood of a subject animal, adding an anti-IL-10 antibody and a Mycobacterium avium subsp. Paratuberculosis antigen to the collected blood followed by culture, and then measuring the amount of produced IFNγ in the blood; a diagnostic method for paratuberculosis characterized in that the amount of produced IFNγ in blood is measured by the IFNγ ELISA method; and a diagnostic method for mycobacterial infection characterized by collecting the blood of a subject animal, adding an anti-IL-10 antibody and a mycobacterial antigen to the collected blood followed by culture, and then measuring the amount of produced IFNγ in the blood.

Claims4

Dependent claims

A cell-mediated immunological diagnostic method for paratuberculosis characterized by comprising:

collecting blood of a subject animal, thereby providing collected blood;
adding (a) an anti-interleukin 10 (IL-10) antibody and (b) a stimulus which induces cell-mediated immunological reaction against Mycobacterium avium subsp. paratuberculosis in the collected blood, to the collected blood, so as to induce said cell-mediated immunological reaction, wherein said stimulus added to the collected blood is a Mycobacterium avium subsp. paratuberculosis antigen;
subsequent to said adding, measuring an amount of produced interferon-γ (IFNγ) in the blood;
subsequent to said measuring, comparing between amounts of the produced interferon-γ (IFNγ) measured in the subject animal and that in a non-infected control animal;
and
distinguishing the subject animal from a non-infected control animal in a case where a statistically significant increase in amounts of produced interferon-γ (IFNγ) is measured in the subject animal.

3 more elements

Dependencies are inferred from the claim text.

Diagnostic method for paratuberculosis

Life of this patent

Diagnostic method for paratuberculosis

Life of this patent

Abstract

Claims4