US 7,731,906 B2 · Processing particle-containing samples

Abstract

A microfluidic device includes an input port for inputting a particle-containing liquidic samples into the device, a retention member, and a pressure actuator. The retention member is in communication with the input port and is configured to spatially separate particles of the particle-containing liquidic sample from a first portion of the liquid of the particle containing fluidic sample. The pressure actuator recombines at least some of the separated particles with a subset of the first portion of the liquid separated from the particles. The device can also include a lysing chamber that receives the particles and liquid from the retention member. The lysing chamber thermally lyses the particles to release contents thereof.

Claims5

Dependent claims

A microfluidic device comprising a microfluidic network, the device comprising:

an input port for receiving a particle-containing liquidic sample;
a retention member in communication with the input port and configured to spatially separate particles of the particle-containing liquidic sample from a first liquidic portion of the particle-containing liquidic sample;
a reservoir in communication with the retention member and configured to receive at least some of the first liquidic portion separated from the particles, wherein a pressure within the reservoir increases upon receiving the first liquidic portion;
and
a gate configured to open a channel downstream of the reservoir thereby decreasing the pressure within the reservoir so that at least some of the separated particles recombine with a subset of the first liquidic portion separated from the particles.

2 more elements

Dependencies are inferred from the claim text.

Facts

Application

10/567,002Filed 2 Aug 2004 · us provisional application

Priority

31 Jul 2003

Published

US 2006/0205085 A114 Sep 2006

Granted

8 Jun 2010

Term ends

Not estimatedThe source records don’t support an estimate.

Classification23 codes

C12Q 1/6806
Involving nucleic acids: preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
B01L 3/502707
By integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g.…
B01L 3/502738
By integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g.…
B01L 3/502753
By integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g.…

Family

Application60/491,269Us provisional application · filed 31 Jul 2003

Application60/551,785Us provisional application · filed 11 Mar 2004

Application60/553,553Us provisional application · filed 17 Mar 2004

US 2006/0205085 A1Pre-grant publication · published 14 Sep 2006

US 7,731,906 B2This patent

From the US filing history. Foreign family members aren’t listed.

Processing particle-containing samples

Life of this patent

Processing particle-containing samples

Life of this patent

Abstract

Claims5