Bioactive materials, methods of making bioactive materials and method of use thereof

A method for the formation of a fibroin suspension, comprising:

• treating a fibroin solution with an agent comprising an acid, an alcohol, a proteolytic enzyme, a biocompatible polymer, or a combination comprising one or more of the foregoing agents, to form a fibroin suspension, and
• mixing core-shell particles into the fibroin suspension, wherein the cores have dimensions from about 1 nanometer to about 500 micrometers in their largest diameter.

A method of tissue construction, comprising administering to a site to be constructed a fibroin solution or suspension in an amount effective to stimulate tissue formation;

• wherein the fibroin solution or suspension is injectable and bioactive,
• wherein the fibroin solution or suspension further comprises a pore-forming particle comprising a core-shell particle, and
• wherein the cores have dimensions from about 1 nanometer to about 500 micrometers in their largest diameter.

A method to induce cell proliferation, comprising contacting a cell with a fibroin solution or suspension in an amount effective to induce cell proliferation;

• wherein the fibroin solution or suspension is injectable and bioactive,
• wherein the fibroin solution or suspension further comprises a pore-forming particle comprising a core-shell particle, and
• wherein the cores have dimensions from about 1 nanometer to about 500 micrometers in their largest diameter.