Immunoassay device with immuno-reference electrode
AssigneeAbbott Point of Care Inc.
InventorsCary James Miller, John Lewis Emerson Campbell
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Abstract
An electrochemical immunosensor system with reduced interference, comprising: a first immunosensor that generates an electrochemical signal based on the formation of a sandwich between an immobilized antibody, a target analyte and a labeled antibody, wherein a portion of the signal arises from non-specific binding of the labeled antibody in the region of the first immunosensor, and a second immunosensor that acts as an immuno-reference sensor and generates a signal that is the same as or predictably related to the degree of non-specific binding which occurs in the region of the first immunosensor, and has an immunocomplex between an immobilized antibody and an endogenous or exogenous protein that is in the sample and that is not the target analyte.
Claims12
An immunosensor system with reduced interference, comprising:
- a first immunosensor that includes a first immobilized antibody and generates a first signal based on a sandwich between the first immobilized antibody, a target analyte and a labeled antibody, wherein a portion of the first signal arises from a degree of non-specific binding of the labeled antibody, and
- a second immunosensor that includes a second immobilized antibody and acts as an immuno-reference sensor and generates a second signal that is the same as or predictably related to the degree of non-specific binding, and has an immunocomplex between the second immobilized antibody and an endogenous or exogenous protein that is in a sample and that is not the target analyte;
- and
- a subtraction analyzer configured to determine a corrected signal from the first and second signals.
Dependencies are inferred from the claim text.