Test system for the determination of in-vivo active hemostasis proteases in biological fluids and/or the usage thereof to determine the in-vivo activation of hemostasis
OwnerThomas W. Stiefinventor
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Abstract
The present invention describes a test system for the determination of in-vivo active hemostasis proteases in biological fluids and the use thereof to determine the in-vivo activation of hemostasis or to diagnose pancreatitis. EDTA and/or EGTA is added to the biological sample to prevent artificial activation of the hemostasis proteases. Arginine and/or guanidine can also be added to the biological sample.
Claims12
A method for the determination of the in vivo activity of a hemostasis protease in blood or plasma comprising the steps of adding EDTA and/or EGTA to a first sample of the blood or plasma in an amount such that hemostasis proteases are not artificially activated prior to detection, incubating the first sample with at least one chromogenic or fluorogenic substrate for the hemostasis protease, the chromogenie or fluorogenic substrate present at a final test concentration of 0.05 mM to 0.8 mM in the first sample, and detecting chromogenic or fluorogenic activity.
Dependencies are inferred from the claim text.