Baselining amplification data
AssigneeBio-Rad Laboratories, Inc.
InventorJeffrey Lerner
Life of this patent
Estimating the term…
Abstract
Methods, apparatus, and systems are provided for processing a data set representing an amplification curve having a baseline portion and a growth portion. Peak objects are generated by taking a derivative of the data set. The first peak object having a value greater than a threshold is identified. An end of the baseline portion from a beginning of the first peak object is estimated.
Claims33
A method of processing a data set representing an amplification curve, the method comprising:
- receiving a set of data points representing a curve having a baseline portion and a growth portion;
- generating a set of one or more peak objects by taking a derivative of at least a portion of the data set, wherein each peak object has a height related to a local maximum of the derivative, wherein the peak objects occur in an order corresponding to an order of the data set;
- identifying, in said order, a first peak object having a value greater than a threshold value;
- and
- estimating an end of the baseline portion from a beginning of the first peak object, wherein the method is implemented using a processor.
An information storage medium having a plurality of instructions adapted to direct an information processing device to perform an operation of processing a data set representing an amplification curve, the operation comprising the steps of:
- receiving a set of data points representing a curve having a baseline portion and a growth portion;
- generating a set of one or more peak objects by taking a derivative of at least a portion of the data set, wherein each peak object has a height related to a local maximum of the derivative, wherein the peak objects occur in an order corresponding to an order of the data set;
- identifying, in said order, the first peak object having a value greater than a threshold value;
- and
- estimating an end of the baseline portion from a beginning of the first peak object.
A PCR detection system comprising:
- a detector for producing a set of data points representing a curve having a baseline portion and a growth portion;
- and
- logic for processing a data set representing an amplification curve to estimate an end of the baseline portion, by:
- generating a set of one or more peak objects by taking a derivative of at least a portion of the data set, wherein each peak object has a height related to a local maximum of the derivative, wherein the peak objects occur in an order corresponding to an order of the data set;
- identifying, in said order, the first peak object having a value greater than a threshold value;
- and
- estimating an end of the baseline portion from a beginning of the first peak object.
Dependencies are inferred from the claim text.