Compositions and methods for inhibiting expression of Eg5 gene
AssigneeAlnylam Pharmaceuticals, Inc.
InventorsDavid Bumcrot, Pamela Tan, Hans-Peter Vornlocher, Anke Geick
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Abstract
The invention relates to a double-stranded ribonucleic acid (dsRNA) for inhibiting the expression of the Eg5 gene (Eg5 gene), comprising an antisense strand having a nucleotide sequence which is less that 30 nucleotides in length, generally 19-25 nucleotides in length, and which is substantially complementary to at least a part of the Eg5 gene. The invention also relates to a pharmaceutical composition comprising the dsRNA together with a pharmaceutically acceptable carrier; methods for treating diseases caused by Eg5 expression and the expression of the Eg5 gene using the pharmaceutical composition; and methods for inhibiting the expression of the Eg5 gene in a cell.
Claims33
A double-stranded ribonucleic acid (dsRNA) for inhibiting the expression of a human kinesin family member 11 (Eg5) gene in a cell, wherein said dsRNA comprises a sense strand comprising a first sequence and an antisense strand comprising a second sequence complementary to SEQ ID NO:1311, wherein said first sequence is complementary to said second sequence and wherein said dsRNA is between 15 and 30 base pairs in length.
A vector for inhibiting the expression of a human kinesin family member 11 (Eg5) gene in a cell, said vector comprising a regulatory sequence operably linked to a nucleotide sequence that encodes at least one strand of a dsRNA, wherein one of the strands of said dsRNA is substantially complementary to SEQ ID NO:1311 and wherein said dsRNA is less than 30 base pairs in length.
At least one vector for inhibiting the expression of a human kinesin family member 11 (Eg5) gene and a VEGF gene in a cell, said vector comprising a regulatory sequence operably linked to a nucleotide sequence that encodes at least one strand of a first dsRNA and at least one strand of a second dsRNA, wherein one strand of said first dsRNA is substantially complementary to SEQ ID NO:1311 and one strand of said second dsRNA is substantially complementary to SEQ ID NO:1242 and wherein said first and second dsRNAs are less than 30 base pairs in length.
Dependencies are inferred from the claim text.