Back to results
US 7,709,456 B2Granted
Modulation of gene expression by oligomers targeted to chromosomal DNA
AssigneeBoard of Regents, The University of Texas System
InventorsDavid R. Corey, David S. Shames, Bethany A. Janowski, John D. Minna
Life of this patent
Estimating the term…
Abstract
Synthesis of a target transcript of a gene is selectively increased in a mammalian cell by contacting the cell with a polynucleotide oligomer of 12-28 bases complementary to a region within a target promoter of the gene under conditions whereby the oligomer selectively increases synthesis of the target transcript.
Claims12
A method of selectively increasing synthesis of a human progesterone receptor transcript in a mammalian cell comprising the steps of:
- (a) contacting the cell with a polynucleotide oligomer of 12-28 bases comprising SEQ ID NO:4, SEQ ID NO:5 or SEQ ID NO:6 under conditions whereby the oligomer selectively increases synthesis of the target transcript;
- and
- (b) detecting resultant selective increased synthesis of the target gene;
- wherein the oligomer is double-stranded RNA.
1 more element
A method of decreasing Cox-2 expression in a cell, the method comprising the steps of:
- (a) contacting the cell with polynucleotide oligomer of 12-28 bases complementary to a region located between nucleotides −100 to +25 relative to a transcription start site of a human progesterone receptor (hPR) gene under conditions whereby the oligomer selectively increases synthesis of hPR;
- and
- (b) detecting decreased synthesis of the Cox-2;
- wherein the oligomer is a double-stranded RNA,
- wherein the target hPR promoter region is −11 to +8.
2 more elements
Dependencies are inferred from the claim text.