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US 7,704,738 B2Granted
Definitive endoderm
AssigneeCythera, Inc.
InventorsKevin Allen D'Amour, Alan D. Agulnick, Emmanuel E. Baetge
Life of this patent
Estimating the term…
Abstract
Disclosed herein are cell cultures comprising definitive endoderm cells and methods of producing the same. Also disclosed herein are cell populations comprising substantially purified definitive endoderm cells as well as methods for enriching, isolating and purifying definitive endoderm cells from other cell types.
Claims12
An in vitro method of producing human definitive endoderm cells, said method comprising:
- obtaining a cell population comprising pluripotent human stem cells;
- and
- providing said cell population with a TGFβ superfamily growth factor and a Wnt family member, thereby generating in said cell population definitive endoderm cells expressing at least SOX17 and HNF3β.
Dependencies are inferred from the claim text.